都梁滴丸提取物的含量测定

Quantitative determination of extract from Duliang Gutta Pills

目的建立成品前都梁滴丸提取物的含量测定方法。方法外标法定量,Kromasil C18色谱柱(4.6 mm×250 mm,5μm),测定阿魏酸的流动相CH3CN与H2O(1 L水中含1mLH3PO4)的体积比为17∶83,检测波长316 nm;测定欧前胡素、异欧前胡素流动相CH3OH与H2O的体积比为70∶30,检测波长300 nm。结果阿魏酸进样量在0.023 4～0.140 6μg范围内,线性关系良好,r=0.999 9,平均回收率为100.4%,RSD为0.39%;欧前胡素进样量在0.016 1～0.322 0μg范围内,线性关系良好,r=0.999 9,平均回收率为102.9%,RSD为0.65%;异欧前胡素进样量在0.004 4～0.087 2μg范围内,线性关系良好,r=0.999 9,平均回收率为100.0%,RSD为0.24%。结论该方法简便、灵敏、准确,可作为都梁滴丸成品前提取物的质量控制方法。

Objective To establish a method for quantitative determination of extract from Duliang Gutta Pills by HPLC.Methods The HPLC procedure was performed on the chromatographic column of Kromasil C18（4.6 mm×250 mm,5 μm）.The mobile phase for ferulic acid was CH3CN-H2O（1 mL of H3PO4 in 1 L of water,17∶83） with the detection wavelength being 316 nm.The mobile phase for imperatorin and isoimperatorin was CH3CN-H2O（70∶30） with the detection wavelength being 300 nm.Results Ferulic acid showed a good linear relationship at a range from 0.023 4 to 0.140 6 μg（r=0.999 9） and the average recovery was 100.4%（RSD=0.39%）.Imperatorin showed a good linear relationship at a range from 0.016 1 to 0.322 0 μg,（r=0.999 9） and the average recovery was 102.9%（RSD=0.65%）.Isoimperatorin showed a good linear relationship at a range from 0.004 4 to 0.087 2 μg（r=0.999 9） and the average recovery was 100.0%（RSD=0.24%）.Conclusion The method was simple,sensitive and accurate,which can be used for the quality control of extract from Duliang Gutta Pills.