左旋精氨酸上调约氏疟原虫感染DBA/2小鼠的抗体应答效应

Antibody Responses Effect in DBA/2 mice by L-Arginine Enhancement during Infection by Plasmodium yoelii 17XL

探讨左旋精氨酸(L-Arginine,L-Arg)对约氏疟原虫(Plasmodium yoelii17XL,P.y17XL)感染DBA/2小鼠体液免疫应答的调节效应。于P.y17XL感染前7 d,连续每日给予DBA/2小鼠L-Arg(1.5 g/kg体重)灌胃预处理,动态观察各组感染小鼠原虫血症水平和生存率;于感染后第0、8和10天分别提取小鼠脾细胞,流式细胞术检测DBA/2小鼠浆细胞分泌IgG1和IgG2a的水平。与NC组比较,L-Arg能够显著降低感染小鼠的原虫血症水平,自愈时间提前。感染后8 d和10 d,L-Arg组CD138+B220+IgG1+细胞数量出现有意义的增加,感染后10 d,L-Arg组CD138+B220+IgG2a+细胞数量明显升高。L-Arg处理可诱导DBA/2小鼠建立更加有效的抗体免疫应答,明显加速DBA/2感染小鼠清除疟原虫。

Body fluid immuno-response regulation effects of L-Arg in DBA/2 in mice during blood-stage infection by P.y17XL were investigated.The mice were orally administrated with L-Arg（1.5 g/kg weight） 7 days continuously before P.y17XL infection（L-Arg group）.Dynamic monitor of plasmodiaemia and survival rate of mice was carried out.Spleen cells of mice were extracted respectively 0 d,8 d,and 10 d after the infection and examined with Giemsa stained thin-smear microscopy and flow cytometry to detect the level of IgG1 and IgG2a of mice splenic cell.The results showed that as compared with NC group（non L-Arg group） the pre-treatment group of mice with L-Arg significantly decreased their plasmodiaemia and shortened self-cure time after infection.And the number of CD138＋B220＋ IgG1＋ and CD138＋B220＋IgG2a＋ cells obviously increased.Therefore,L-Arg treatment could induce DBA/2 mice to establish antibody responses more effectively and significantly accelerate the removal of P.y17XL in infected mice.