石参总黄酮抗氧化活性研究

Antioxidant Effect of Flavonoids from Uraria crinita

目的:研究石参总黄酮的抗氧化活性。方法:采用50%乙醇回流冷凝提取石参根,提取液分别用乙醚和乙酸乙酯萃取,得到石参总黄酮。利用2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐[2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate),ABTS]法检测总抗氧化能力,采用分光光度法检测对1,1-二苯基-2-苦味肼基(1,1-Diphenyl-2-picrylhydrazy,DPPH),羟基自由基(.OH)、一氧化氮(NO)的清除作用及对脂质过氧化的抑制作用。结果:石参总黄酮在20～100 mg·L-1对DPPH,ABTS+,.OH和NO等自由基具有明显的清除作用,最大清除率分别达到60.89%,74.87%,41.77%,81.01%;对脂质过氧化亦有明显的抑制作用,最大抑制率达41.07%,并具有浓度依赖性。结论:石参总黄酮具有较好的清除自由基活性和抑制脂质过氧化的作用。

Objective：To investigate the radical scavenging effect of flavonoids from U.crinita.Method： Flavonoids were obtained from U.crinita root by extraction with 50% ethanol,ether and ethyl acetate.Total antioxidant activity was investigated by 2,2′-Azinobis-（3-ethylbenzthiazoline-6-sulphonate）（ABTS）.The abilities of scavenging 1,1-diphenyl-2-picrylhydrazy（DPPH）,·OH and NO,as well as the inhibitory effect of lipid peroxidation were studied through ultraviolet spectrophotometry in vitro.Result： Flavonoids from U.crinita,ranging from 20-100 μg·mL-1,sharply scavenged the DPPH,ABTS＋,·OH,NO,and inhibited lipid peroxidation dose dependently.Conclusion： Flavonoids from U.crinita shows potent ability in scavenging radicals and inhibiting lipid peroxidation.