蜈蚣提取物对小鼠宫颈肿瘤生长的影响及其作用机制的实验研究

Effect of Centipede Extract on Cervical Tumor of Mice and Its Mechanism

目的:研究蜈蚣提取物在体内对宫颈肿瘤生长的影响及其作用机制。方法:观察不同浓度、不同蜈蚣提取物对荷瘤小鼠的生存期、抑瘤率、免疫功能等方面的影响以研究其体内抗肿瘤效应,并探索其作用机制。结果:两种蜈蚣提取物(CE、CA)处理荷瘤鼠,实验中无一死亡,抑瘤率分别可达52.85%、33.65%;光镜观察实体瘤组织发现盐水对照组瘤组织浸润横纹肌组织中生长旺盛,而药物处理组则普遍可见大量坏死和凋亡;电子透射显微镜观察超微结构发现实验组大量核固缩、边聚的凋亡肿瘤细胞;免疫组化结果显示CE和CA使Bax表达量明显增高,Bc l-2和Survivin表达量降低,但Sm ac的表达无显著差异;CE和CA对VEGF的含量影响不大;胸腺指数和脾脏指数无显著改变;CE组小鼠CD3+的T细胞总数无明显改变,CD4+/CD8+比值、CD19+的B细胞数下降;CA组CD3+以及CD4+细胞下降显著;肝、肾组织切片发现CE、CA在本文实验浓度范围内无明显毒副作用。结论:两种蜈蚣提取物(CE、CA)均可在小鼠体内抑制宫颈肿瘤的生长,通过Bax和Caspase-3介导的线粒体信号转导途径诱导肿瘤组织凋亡机制可能参与了其体内抗肿瘤效应。

To study the anti-tumor activity of centipede extract on cervical tumor of mice and its mechanism.Methods： The tumor-bearing mice were treated with centipede extract from two solvents [ether（CE） and alcohol（CA）] at different comcentration.The mice′ life span,tumor inhibition rate and immune function were estimated.Results： No mice died in CE and CA treatment groups and the tumor inhibition rate was 52.85% and 33.65% respectively.Observed the tumor tissue slices with light microscope and found infiltration of tumor cells in striated muscle in the control group but centipede treatment groups had massive necrosis and apoptosis.Karyopyknosis and apoptotic tumor cells were observed in the treatment groups under transmission electron microscopy.Compared with control group,the expression of Bax increased,the expressions of Bcl-2 and Survivin decreased,but the content of VEGF,the indexes of thymus and spleen had no significant change in treatment groups.The number of CD3＋ T lymphocytes had no significant change while the ratio of CD4＋ and CD8＋,the number of CD19＋ B lymphocytes decreaed in the CE group.The numbers of CD3＋ and CD4＋ lymphocytes decreased in the CA group.The pathological examine indicated no obvious change in the tissue slices of mice′s liver and kidney,manifested the concentrations of CE and CA between the article′s had no visible side effect.Connclusion： The two extracts（CE and CA） can suppress the growth of cervical tumor and its mechanism may be related to Bax and Caspase-3 medicated the mitochondrial signal transit pathway.