溪黄草根不同溶剂提取物的抗氧化性

Antioxidant Activity of Different Solvent Extracts from Rabdosia serra(Maxim.) Hara Roots

考察7种溶剂(蒸馏水、60%乙醇、无水乙醇、甲醇、丁醇、氯仿和乙酸乙酯)对溪黄草根中可溶出成分的提取效果,研究7种提取物总酚含量、清除DPPH自由基能力、清除ABTS+.能力和螯合Fe2+的能力。结果表明:水和60%乙醇对溪黄草根中可溶出成分的提取效果最好;60%乙醇提取物总酚含量最高,清除DPPH自由基能力最高(超过迷迭香提取物、竹叶提取物、抗坏血酸棕榈酸酯),清除ABTS+.能力最好(超过迷迭香提取物),并具有较强的螯合Fe2+能力(超过迷迭香提取物、竹叶提取物、抗坏血酸棕榈酸酯)。

The extraction efficiency of soluble components from Rabdosia serra（Maxim.） Hara roots using seven kinds of solvents such as distilled water,60% ethanol,aqueous ethanol,methanol,butanol,chloroform and ethyl acetate was explored.Meanwhile,total phenol contents and the abilities to scavenge DPPH and ABTS free radicals and to chelate Fe2＋ of seven different extracts were also evaluated.The results showed that water and 60% ethanol extracts had better effectiveness in extracting soluble components from R.serra（Maxim.） Hara roots.In addition,the highest content of total phenols was detected in 60% ethanol extract.Moreover,60% ethanol extract also revealed the best DPPH racial scavenging capacity（better than rosemary extract,bamboo extract and ascorbyl palmitate）,the best ABTS＋· scavenging capacity（better than rosemary extract）,and the best Fe2＋ chelating capacity（better than rosemary extract,bamboo extract and rutin）.