三七总皂苷联合三苯氧胺对乳腺癌T47D细胞生长与凋亡影响

Impact of Panax Notoginsenosidum and Tamoxifen on Breast Cancer T47D Cell

目的:探讨中药三七总皂苷和TAM联合应用对乳腺癌T47D细胞生长与凋亡的影响。方法:分别或联合应用不同浓度的TAM、三七总皂苷作用于T47D细胞,应用四甲基偶氨唑蓝(MTT)比色法分析对细胞生长抑制作用。应用流式细胞技术测定细胞周期分布和凋亡率。结果:TAM及三七总皂苷均可有效抑制乳腺癌T47D细胞生长,抑制作用随浓度增加和时间延长而增强,三苯氧胺(1.5×10-5mol/L)与三种浓度三七总皂苷联合与单药应用相比均可显示出明显的抑制优势;两者均可使T47D细胞周期中S期细胞含量较空白对照组明显降低;联用方案显示出明显促进细胞凋亡优势。结论:三七总皂苷对乳腺癌细胞有直接抑制作用,该作用具有时间及浓度依赖性;高浓度三七总皂苷与TAM联合可以增强TAM对乳腺癌细胞的抑制作用,其作用机制与影响细胞周期、协同促进凋亡有关。二者的联合应用会使抗肿瘤作用增强。

Objective：To investigate impact of Panax Notoginsenosidum and Tamoxifen （TAM） on breast cancer T47D Cell.Methods：Different concentration of TAM and Panax Notoginsenosidum were administered to T47D Cell.The cell growth inhibition was assessed by MTT,the cell cycle distribution and apoptosis rate were assessed by FCM.Results：Panax Notoginsenosidum and Tamoxifen （TAM） can respectively inhibit growth of T47D cell,positively correlated with higher concentration and increasing time.TAM 1.5×10-5mol/L on top of 3 different concentration of Panax Notoginsenosidum had more significant inhibition than used alone.Both can reduce amount of cell at cycle S in comparison with blank control group.Moreover,combined use had the tendency to stimulate apoptosis.Conclusion：Panax Notoginsenosidum has direct,time and concentration dependent inhibition effect on breast cancer T47D Cell.High dose of Panax Notoginsenosidum can enhance anti-tumor effect of TAM by impacting cell cycle and apoptosis.