AB-8型大孔吸附树脂分离纯化大叶金花草总黄酮

Using AB-8 Macroporous Adsorption Resin for Separation and Purification of Total Flavonoids from Stenoloma chusana(L.) Ching

目的:研究AB-8型大孔吸附树脂分离纯化大叶金花草总黄酮的工艺参数,为工业化生产提供依据。方法:通过静态、动态相结合的方法,确定最佳工艺参数。结果:最佳工艺参数为上样液pH4.5、上样液质量浓度1.00mg/mL、上样液流速80mL/h、洗脱液为体积分数70%乙醇溶液、洗脱液流速40mL/h、洗脱液用量60mL,分离纯化后的总黄酮产品纯度可达66.16%。结论:采用AB-8型大孔吸附树脂分离纯化大叶金花草总黄酮操作简单、安全、成本低廉,有较高的应用价值。

Objective： To establish optimal parameters for the separation and purification of total flavonoids from the whole plant of Stenoloma chusana（L.） Ching using AB-8 macroporous adsorption resin.Methods： Static and dynamic adsorption and desorption behaviors of AB-8 macroporous adsorption resin were evaluated for achieving the best purification of total flavonoids.Results： The optimum adsorption and desorption parameters were found to be： sample pH 4.5,sample concentration 1.00 mg/mL,sample loading flow rate 80 mL/h,70% ethanol as the eluent,desorption flow rate 40 mL/h and eluent amount 60 mL,resulting in a total flavonoid purity of 66.16%.Conclusion： AB-8 macroporous adsorption resin is safe,economical and easy to use and can therefore be promisingly applied to separate and purify total flavonoids from the whole plant of Stenoloma chusana（L.） Ching.