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Comparison of Osteogenesis Between Two Kinds of Stem Cells from Goat Combined Calcium Phosphate Cement in Tissue Engineering 被引量：1

Comparison of Osteogenesis Between Two Kinds of Stem Cells from Goat Combined Calcium Phosphate Cement in Tissue Engineering
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摘要 To explore the possible mechanism of osteogenesis for deciduous teeth stem cells (DTSCs) in vivo/ vitro, stem cells from goat deciduous teeth (SGDs) were firstly isolated, induced and transplanted into immunocompromised mice. The SGDs's mineralization pattern and osteogenesis were compared with bone marrow messenchymal stem cells (BMMSCs) from goats. SGDs have similar osteogenic differentiation pattern in vitro and bone-like tissue formation mechanism in vivo to BMMSCs; moreover SGDs have stronger alkaline phosphatase (ALP) gene expression and osteopontin (OPN) gene expression levels than BMMSCs; also SGDs can form more bone-like tissues than BMMSCs when cell-scaffold compounds are transplanted into immunocompromised mice. This pre-clinical study in a large-animal model confirms that DTSCs may be an appropriate source of stem cells in repairing bone defects with tissue engineering. To explore the possible mechanism of osteogenesis for deciduous teeth stem cells （DTSCs） in vivo/ vitro, stem cells from goat deciduous teeth （SGDs） were firstly isolated, induced and transplanted into immunocompromised mice. The SGDs＇s mineralization pattern and osteogenesis were compared with bone marrow messenchymal stem cells （BMMSCs） from goats. SGDs have similar osteogenic differentiation pattern in vitro and bone-like tissue formation mechanism in vivo to BMMSCs; moreover SGDs have stronger alkaline phosphatase （ALP） gene expression and osteopontin （OPN） gene expression levels than BMMSCs; also SGDs can form more bone-like tissues than BMMSCs when cell-scaffold compounds are transplanted into immunocompromised mice. This pre-clinical study in a large-animal model confirms that DTSCs may be an appropriate source of stem cells in repairing bone defects with tissue engineering.
作者 赵伟 陆家瑜 郝永明 张秀丽 瞿晓辉 华丽 曹春花 邹德荣
机构地区 Department of Stomatology Oral Bioengineering Laboratory/Oral Tissue Engineering Laboratory
出处 《Journal of Shanghai Jiaotong university(Science)》 EI 2011年第5期628-635,共8页 上海交通大学学报（英文版）
基金 the Science and Technology Commission fund of Shanghai Municipality (No. 09JC1411700) the Collaboration Projects of Development and Research from Basic Science of Stomatology of Shanghai(No. S30206-KF09) the Fund of Shanghai Jiaotong University School of Medicine (No. 09XJ21030)
关键词 stem cells from goat deciduous teeth (SGDs) MINERALIZATION OSTEOGENESIS bone marrow mesenchymal stem cells (BMMSCs) tissue engineering stem cells from goat deciduous teeth （SGDs）, mineralization, osteogenesis, bone marrow mesenchymal stem cells （BMMSCs）, tissue engineering
分类号 R782.1 [医药卫生—口腔医学]
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Journal of Shanghai Jiaotong university(Science)
2011年 第5期

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