广州地区贝类中人类杯状病毒感染调查研究

Infectious Investigational Research of Human Calicivirus in Shellfish of Guangzhou

从2007年10月-2009年9月，在广州市水产品市场收集127个牡蛎、94个毛蚶、342个花蛤、265个带子，采用荧光定量PCR方法检测人类杯状病毒HuCV的感染，旨在为贝类的安全监控提供基础研究资料。研究结果表明：牡蛎的HuCV阳性检出率最高，为24.4l％（31／127）；毛蚶和带子样品中HuCV，4-阳性率分别为11．70％（11／94）和1．89％（5／265）；而花蛤中未检出HuCV。牡蛎中存在着人类杯状病毒不同基因型的混合感染。结果还显示贝类中的HuCV感染一般在秋冬季节流行：在2007年10月～2008年2月和2008年10月-2009年2月期间，牡蛎中HuCV总检出率分别为23．08％（12／52）和35．85％（19／53）；而在2008、2009年的3月～9月间，牡蛎中均未发现HuCV感染。

Epidemic and infect of human calieivirus were monitored in fishery product markets of Guangzhou during October 2007-September 2009, including samples of 127 oyster, 94 anadara subcrenata, 342 meretrix, 265 belt bivalves. Infect of human calicivirus was detected by real-time fluorogenic quantitative PCR, aiming to provide fundamental data for shellfish safety monitoring. Results showed that the positive rate of HuCV was highest in oyster, was 24.41% （31/127） ; The HuCV positive rate were 11.70 % （11/94） and 1.89 % （5/265） in anadara suberenata and belt bivalves respectively; no positive samples were detected in meretrix. Different genetypes of HuCV infect were found in oyster; Furthermore, the epidemic season of HuCV happens mostly in autumn and winter, from Oetober 2007 to February 2008 and from September 2008 to February 2009, the HuCV positive rate were 23.08 % （12/52） and 35.85 % （19/53）in oyster respectively; but during March- September of 2007and 2008, the HuCV positive rate was 0.