复方玄驹胶囊对去势雄性大鼠性激素水平及性器官重量的影响

Compound Xuanju Capsule improves sex hormones and sex organ indexes in castrated male rats

目的:探讨复方玄驹胶囊对去势雄性大鼠性激素水平及性器官重量的影响。方法:采用随机分组、空白对照、模型对照的临床研究方法,从60只SPF级雄性幼年SD大鼠中,随机取出12只大鼠为正常对照组,余大鼠去势(摘除双侧睾丸)后,随机均分4组,分别为模型对照组、复方玄驹胶囊高、中、低剂量组。正常模型对照组予生理盐水灌胃,模型组采用复方玄驹胶囊配制液灌胃干预,20 d后,采用放射免疫法检测各组大鼠外周血液中睾酮(T)、黄体生成素(LH)和卵泡刺激素(FSH)水平,同时称取附睾、包皮腺、精囊腺、前列腺及提肛肌的重量。结果:①与正常对照组相比,去势各组T水平显著降低,说明造模成功。与模型对照组相比,复方玄驹胶囊高、中、低剂量组T水平明显升高,差异有统计学意义(P<0.01)。②与正常对照组相比,模型对照组、复方玄驹胶囊各剂量组LH水平显著升高,差异有统计学意义(P<0.05);FSH水平虽有所升高,但差异无统计学意义。③与正常对照组相比,模型对照组包皮腺、精囊腺、前列腺、提肛肌指数明显降低,说明造模成功;与模型对照组相比,复方玄驹胶囊高剂量组精囊腺指数明显提高,差异有统计学意义(P<0.05);而复方玄驹胶囊不同剂量组之间比较,包皮腺、前列腺、提肛肌指数均未见明显增加,差异无统计学意义(P>0.05)。结论:复方玄驹胶囊可改善雄性SD大鼠外周血中激素水平,提高附性器官指数,可能是临床治疗ED的重要作用机制之一。

Objective： To investigate the effects of Compound Xuanju Capsule on the levels of sex hormones and the weight of sexual organs in castrated male rats.Methods： A randomized model control trail was performed in 60 young male SD rats of SPF grade,of which 12 were included in the normal control group,and the others were castrated and randomly divided into a model control group and a high-dose,a median-dose and a low-dose Xuanju group.The control groups received intragastric administration of normal saline,and the model groups solution of Compound Xuanju Capsule,all for 20 days.Then we determined by radioimmunoassay the levels of testosterone（T）,luteinizing hormone（LH） and follicle stimulating hormone（FSH） in the peripheral blood of the rats,and measured the weights of the epididymis,preputial gland,seminal vesicle,prostate and levator ani muscle.Results： The T levels were remarkably lower in the castrated groups than in the normal controls,and significantly higher in the three Xuanju groups than in the model controls（P 0.01）.Both LH and FSH levels were increased in the model control and Xuanju groups as compared with the normal control group,the former with statistically significant difference（P 0.05） and the latter without.In comparison with the normal controls,the model control rats showed a marked reduction in the indexes of the preputial gland,seminal vesicle,prostate and levator ani muscle,and the high-dose Xuanju group exhibited a significant increase in the seminal vesicle index as compared with the model controls（P0.05）.There were no statistically significant differences in the indexes of preputial gland,prostate and levator ani muscle among different dose groups（P0.05）.Conclusion： Compound Xuanju Capsule can elevate T and LH levels in the peripheral blood of male SD rats and improve the indexes of their sex organs,which may be an important mechanism behind its effect on ED.