半夏超临界CO_2乙醇萃取物抗惊厥作用的实验研究

Study of supercritical-CO_2 alcohol fluid extractions of Pinellia tuber on anticonvulsant effect

目的:研究半夏超临界CO2乙醇萃取物对不同惊厥动物模型的对抗作用,并探讨其作用机制。方法:采用最大电休克(MES)和戊四唑惊厥模型(MET),以托吡酯(TPM)为阳性对照,观察其抗惊厥作用;建立大鼠皮层定位注射青霉素点燃模型,观察其灌胃给药对惊厥行为和脑电图的影响。结果:半夏超临界CO2萃取物对MES模型有对抗作用,且量效呈正相关性。可延长MET惊厥潜伏期(P<0.01),对抗MET发作。对青霉素点燃模型亦产生对抗作用,可延长痫性发作潜伏期、减轻发作程度,延长痫性放电的潜伏期,减少痫波发放频率,减小放电最高波幅,与模型组比较,均具有显著性差异(P<0.05,P<0.01)。结论:半夏超临界CO2乙醇萃取物可剂量依赖性对抗小鼠最大电休克惊厥,对抗戊四唑惊厥,也能对抗大鼠皮层定位注射青霉素点燃发作,其抗惊厥作用性质和托吡酯相似,而抗青霉素点燃发作较托吡酯强。

Objective： To investigate the antagonize action of supercritical-CO2 alcohol fluid extraction of Pinellia tuber（SFE-CO2 PT） on different convulsive model and explore its mechanism of anticonvulsant action.Method： Maximal electroshock seizure（MES） and Metrazol seizure test（MET） were used to analyze the anticonvulsant effects in mice given by ig SFE-CO2 PT.Using the model of seizure rats induced by penicillin localized injected in cortex investigated the effect of SFE-CO2 PT on the convulsant behaviors and hippocampus EEG.Result： SFE-CO2 PT could dose-dependently antagonize MET modle,and the dose-effect was positively correlated.It could be extended MET convulsions incubation period（P0.01） and against the attack of MET.It had antagonism effect to the model of seizure rats induced by penicillin localized injected in cortex,extended incubation period of seizure,reduced the extent of attack,extended incubation period of epileptic discharges,reduced the frequency of epileptic waves release,reduced the maximum amplitude of the discharge,which compared with model group,all of them had significant differences（P0.05,P0.01）.Conclusion： The SFE-CO2 PT can dose-dependently antagonize MES and MET modle,meanwhile,it can against the attack of seizure rats induced by penicillin localize injected in cortex.The anticonvulsant effect is similar to Topamax modle,but compared with Topamax modle,the MES and MET modle is stronger while the seizure rats induced by penicillin localize injected in cortex is weaker.