腹主动脉瘤组织HMGB1与RAGE表达及意义

THE EXPRESSIONS OF HMGB1 AND RAGE AND THEIR CLINICAL SIGNIFICANCE IN ABDOMINAL AORTIC ANEURYSM

目的检测高迁移率族蛋白B1（HMGB1）及晚期糖基化终产物受体（RAGE）在腹主动脉瘤（AAA）组织中的表达,探讨它们在AAA发展、破裂中的作用。方法 AAA病人42例,小AAA（横径〈5 cm）病人9例,中AAA（横径5~7 cm）病人15例,大AAA（横径〉7 cm）病人6例,破裂AAA病人12例;另选10例正常腹主动脉壁组织作为对照。采用免疫组化方法检测两组HMGB1及RAGE蛋白的表达。结果 AAA组织中HMGB1、RAGE的阳性表达率均明显高于正常腹主动脉组织（2χ=15.61、13.37,P〈0.01）,大、中AAA组织中RAGE的阳性表达率高于小AAA组织（χ2=6.075,P〈0.05）,大AAA和破裂AAA组织中RAGE的阳性表达率高于中小AAA组织（2χ=4.978,P〈0.05）。而HMGB1在不同大小AAA及破裂AAA组织中的表达差异无显著性（χ2=3.060,P〉0.05）。HMGB1与RAGE在AAA组织中的表达无相关性（P〉0.05）。结论 HMGB1可能参与了AAA的发展过程,尤其RAGE与AAA的扩张和破裂可能密切相关。

Objective To detect the expressions of HMGB1 and RAGE in abdominal aortic aneurysm（AAA） and their role in the development and rupture of the disease. Methods This study consisted of 42 patients with AAA,of those,nine were small AAA（transverse diameter（TD） 5 cm）,15 moderate（TD 5-7 cm）,six large（TD7 cm）,and 12 with rupture AAA.Ten abdominal aortic wall specimens were collected to be served as controls.The expressions of HMGB1 and RAGE in AAA and normal controls were measured by using immunohistochemistry method. Results The positive expression rates of HMGB1 and RAGE in AAA were significantly higher than that in normal control group（χ2=15.61,13.37;P0.01）.The positive RAGE expression in large and moderate AAA was higher than that in small AAA（χ2=6.075,P0.05）,and that in large AAA and rupture AAA were higher than that in small and moderate ones（χ2= 4.978,P0.05）,whereas the expressions of HMGB1 in various-size AAA and rupture AAA were no significant differences（χ2=3.060,P0.05）.No correlation between the expressions of HMGB1 and RAGE were observed in AAA tissues（r=0.189,P0.05）. Conclusion Both HMGB1 and RAGE may involve in the process of AAA development,and RAGE,in particular,is closely related with dilatation and rupture of AAA.