摘要
IM To determine whether antisense insulinlike growth factorI(IGFI) gene can modulate CEA and AFP expression in human hepatoma cells (HepG2).METHODS Transfection of HepG2 cells was accomplished using Lipofectin reagent. Northern blot analysis confirmed the antisense IGFI RNA of the transfected cells. CEA and AFP levels were measured using radioimmunoassay.RESULTS Human hepatoma cell lines (HepG2) were transfected with antisense IGFI gene. Northern blot analysis confirmed that antisense IGFI RNA was expressed in the transfected cells. The effect of antisense IGFI gene on CEA and AFP expression was demonstrated by the fact that the CEA and AFP levels in the supernatant of transfected cell culture were significantly lower as compared with the parent cells, 〔CEA 70μg/L±076μg/L and 329μg/L±180μg/L (P<005) and AFP 5363μg/L±602μg/L and 90μg/L±526μg/L (P<001), respectively〕.CONCLUSION The malignant potentiality of the transfected cells was partially suppressed. Antisense IGFI gene can modulate the expression of CEA and AFP in human hepatoma cell lines (HepG2).
ＣＥＡａｎｄＡＦＰｅｘｐｒｅｓｓｉｏｎｉｎｈｕｍａｎｈｅｐａｔｏｍａｃｅｌｓｔｒａｎｓｆｅｃｔｅｄｗｉｔｈａｎｔｉｓｅｎｓｅＩＧＦＩｇｅｎｅＺＨＡＮＧＬｉ１，ＬＩＳｈｕＮｏｎｇ２ａｎｄＷＡＮＧＸｉａｏＮｉｎｇ１Ｓｕｂｊｅｃｔｈｅａｄｉｎｇｓｉ...
