摘要
This paper reports the use of an electrochemically polymerized Toluidine Blue (TB) film electrode.The film on platinum electrode surface was analyzed with ESCA.The heterogeneous electron transfer processes of myoglobin at the polymerized TB film electrode have been investigated using in situ UV-visible spectroelectrochemistry.The formal potential(E°′)and electron transfer number(n)of myoglobin were calculated as E°′=0.045 V(vs.NHE)and n=0.99.The exhaustive reduction and oxidation electrolyses are achieved in 130 s and 110 s respectively,during a potential step between-0.4 V and+0.4 V.A formal heterogeneous electron transfer rate constant(ksh)of 1.09× 10^(-4) cm/s and a transfer coefficient(α)of 0.47 were obtained by cyclic voltabsorptometry,which indicated that myoglobin underwent a quasi-reversible electrode process at the polymerized TB film electrode.
This paper reports the use of an electrochemically polymerized Toluidine Blue (TB) film electrode.The film on platinum electrode surface was analyzed with ESCA.The heterogeneous electron transfer processes of myoglobin at the polymerized TB film electrode have been investigated using in situ UV-visible spectroelectrochemistry.The formal potential(E°′)and electron transfer number(n)of myoglobin were calculated as E°′=0.045 V(vs.NHE)and n=0.99.The exhaustive reduction and oxidation electrolyses are achieved in 130 s and 110 s respectively,during a potential step between-0.4 V and+0.4 V.A formal heterogeneous electron transfer rate constant(ksh)of 1.09× 10^(-4) cm/s and a transfer coefficient(α)of 0.47 were obtained by cyclic voltabsorptometry,which indicated that myoglobin underwent a quasi-reversible electrode process at the polymerized TB film electrode.
基金
Project supported by the National Natural Science Foundation of China
