摘要
A method for the analysis of monosaccharides derived from glycoproteins and glycolipids was described using the newly developed technique of capillary zone electrophoresis(CZE).It is found that,at ca.0.5-5 nL in- jection,CZE can offer a high resolution of most monoses from glycoprotein and glycolipids.A mixture of amino-and non-aminomonosaccharides,which is difficult to separate with chromatographic methods,was separated complet- ely by CZE in borate buffer after the monosaccharides were derivatized with 1-naphthylamine.
A method for the analysis of monosaccharides derived from glycoproteins and glycolipids was described using the newly developed technique of capillary zone electrophoresis(CZE).It is found that,at ca.0.5-5 nL in- jection,CZE can offer a high resolution of most monoses from glycoprotein and glycolipids.A mixture of amino-and non-aminomonosaccharides,which is difficult to separate with chromatographic methods,was separated complet- ely by CZE in borate buffer after the monosaccharides were derivatized with 1-naphthylamine.
基金
This project is supported by National Natural Sience Foundation of China
