牙周致病菌在动脉粥样硬化斑块中的检测

Detection of five periodontal pathogens in coronary atherosclerotic plaque

目的检测冠状动脉粥样硬化斑块中的5种特异性牙周致病菌。方法收集101例行冠状动脉搭桥手术患者的动脉粥样硬化斑块标本，采用Chelex-100法提取冠状动脉粥样硬化斑块中的DNA，并通过PCR分别检测动脉粥样硬化斑块中的牙龈卟啉单胞菌（Porphyromonas gingivalis，Pg）、伴放线放线杆菌（Actinobacillus actinomycetemcomitans，Aa）、具核梭杆菌（Fusobacterium nucleatum，Fn）、中间普氏菌（Prevotella intermedia，Pi）、福赛斯坦纳菌（Tannerella forsythensis，Tf）5种牙周特异性致病菌。结果101例患者动脉粥样硬化斑块中牙龈卟啉单胞菌（Pg）的检出率为31％，福赛斯坦纳菌（Tf）为42％，中间普氏菌（Pj）为26％，具核梭杆菌（Fn）为21％，伴放线放线杆菌（Aa）为23％。这几种细菌的PCR产物通过测序，结果与GenBank数据库中的序列进行比对同源性达99％～100％。结论牙周致病菌在冠心病的发生、发展中可能发挥着一定作用，考虑慢性牙周炎与冠心病之间有一定的相关性。

Objective To detect five periodontal pathogenic bacteria in coronary atherosclerotic plaques. Methods Atheroselerotic plaque specimens were obtained from 101 patients who scheduled for coronary artery bypass graft surgery. The bacteria DNA was obtained from coronary atherosclerotic plaques using the chelex-100 method. The extracted DNA were examined by PCR. Results Within the 101 pieces of atherosclerotic plaque samples Porphyromonas gingivalis （ Pg, 31% ） , Tannerella forsythensis （ Tf, 42% ） , Prevotella intermedia （ Pi, 26% ） , Fusobacterium nucleat um （ Fn, 21% ） , Actinobacillus actinomycetemcomi- tarts（ Aa, 23% ）. PCR products were sequenced and were compared with GenBank sequences, the homology was 99%-100%. Conclusion Periodontitis might affect the development of atherosclerosis and there is a correlation between coronary heart disease and chronic periodontitis.