人颅咽管瘤裸鼠皮下移植瘤模型的初步建立

Establishment of subcutaneous xenotransplanted tumor model of human craniopharyngioma in nude mice

目的初步建立人颅咽管瘤(CP)的裸鼠皮下移植瘤模型。方法采用胰蛋白酶消化法将新鲜颅咽管瘤组织进行原代细胞培养,然后将纯化的第3代颅咽管瘤细胞接种于到BALB/c裸小鼠背部皮下。观察接种成瘤后瘤组织生长情况和特点,明确肿瘤性质。结果 CP细胞可以在裸鼠皮下存活,接种后8d可见有移植瘤形成,成瘤率为33.3%,病理切片显示肿瘤样鳞状上皮细胞团增生。结论利用颅咽管瘤细胞初步建立了人颅咽管瘤裸鼠皮下移植瘤模型,为进一步研究颅咽管瘤的生长特性奠定了基础。

Objective To establish a preliminary subcutaneous xenotransplanted tumor model of human craniopharyngioma in nude mice.Methods Craniopharyngioma cells were primaryly cultured by trypsin digestion.Then,the purified cells in third passage were implanted subcutaneously into the nude mice.The specimens were investigated by microscope.Results Craniopharyngioma cells could survive in the nude mice.The xenograft could be found on 8 d after implanting.The tumor formation rate was 33.3%.The disorder growth of the squamous epithelial tumor cells could be subcutaneously found with microscope in nude mice.Conclusion The subcutaneously xenotransplanted tumor model of human craniopharyngioma in nude mice is successfully established by the craniopharyngioma cells,which may benefit to further studies on this tumor.