木薯腋芽萌发及生长影响因素的研究

Study on Several Factors Influencing Sprouting and Growth of Axillary Bud of Cassava

以木薯(Manihot esculenta)优良品种辐选01作为材料,研究了不同浓度的NaClO溶液、不同培养基配方、赤霉素和维生素C浸泡外植体对木薯腋芽萌发及生长的影响。结果表明,木薯嫩茎段宜用质量分数2%的NaClO溶液消毒10 min,老茎段宜用质量分数10%的NaClO溶液消毒12 min;适宜的培养基为MS+1.0 mg/L 6-BA+0.1%活性炭,腋芽萌发的效果较好;接种前用维生素C浸泡外植体能有效减少木薯组织褐变;用赤霉素浸泡处理有利于加速木薯腋芽的萌发和生长。

The effects of NaC10 concentration, media formula, GA3 and vitamin C on sprouting and growth of axillary bud of cassava （Manihot esculenta） were studied using cassava cutlivar Fuxuan 01 as material. The results showed that tender stems should be sterilized by 2% NaC10 solution for 10 min; While old stems should be treated by 10% NaC10 solution for 12 min. The MS＋ 1.0 mg/L 6-BA＋0.1% activated carbon was the suitable medium for shoot induction as the sprouting rate and budding quality was high. Browning rate of cassava explants could be reduced by immersing them in vitamin C before culture. GA3 treatment before inoculation could promote the sprouting and growth of axillary bud of cassava.