广痛消泡沫气雾剂止痛促愈合的实验研究

Experimental Study on Pain Relieving and Promoting Healing Efficacy of Guangtongxiao Foam Aerosol

目的观察广痛消泡沫气雾剂对小鼠的镇痛作用及对大鼠创面的促愈合作用。方法扭体实验将小鼠随机分为模型组、正常组、强痛定组和广痛消大、中、小剂量组,先予腹腔注射药物,0.5 h后再次腹腔注射0.6%冰醋酸10 mL/kg,观察末次给药后0.5 h内小鼠扭体次数、扭体潜伏时和扭体反应抑制率。热板实验将雌性小鼠随机分为盐水组、强痛定组和广痛消大、中、小剂量组,给药前1 h及给药后0.5 h后测定痛阈值。愈合实验将大鼠分为广痛消组、盐水组和康复新组,用打孔器在大鼠背部制造创面,每日换药1次,至创面完全愈合,观察第3、7、14日创面愈合率及总愈合时间。结果广痛消大、中、小剂量组扭体次数均明显少于模型组和强痛定组(P<0.05),扭体潜伏时均长于模型组和强痛定组(P<0.05);广痛消大、中剂量组痛阈提高百分率均大于盐水组(P<0.05),与强痛定组比较差异无统计学意义(P>0.05)。广痛消组创面愈合率第14日高于盐水组(P<0.05),与康复新组比较差异无统计学意义(P>0.05);愈合时间较盐水组短(P<0.05),与康复新组比较差异无统计学意义(P>0.05)。结论广痛消泡沫气雾剂对小鼠有较好的镇痛作用,对大鼠创面有较强的促愈合作用,剂量越大,疗效越好。

Objective To study the pain relieving efficacy of Guangtongxiao（GTX） foam aerosol on mice and promoting healing efficacy on rats.Methods The mice were divided into model group,normal group,bucinperazine group,GTX high-dose group,GTX medium-dose group and GTX small-dose group in the writhing test.The mice were intraperitoneally injected corresponding drugs and 0.6% acetic acid half an hour later,then the writhing numbers,writhing latency and writhing inhibition proportion were observed.In the hot-plate test,the mice were divided into saline group,bucinperazine group,GTX high-dose group,GTX medium-dose group and GTX small-dose group.The pain threshold was observed one hour before administration and half an hour after administration.Rats were divided into GTX group,saline group and Kangfuxin group in studying the promoting healing efficacy.Made wound on the back of rat with puncher and changed dressing every day.The wound healing rate on 1st,7th,14th day and wound healing time were observed.Results The writhing numbers of GTX large-,medium-and low-dose group were all less than the model group and bucinperazine group（P〈0.05）,the writhing latency of GTX large-,medium-and low-dose group were all longer than the model group and bucinperazine group（P〈0.05）.The increases of the percentage of pain threshold of GTX large-and medium-dose groups were higher than saline group,but had no significant differences compared with bucinperazine group（P〈0.05）.On the fourteenth day,the wound healing rate of GTX group was higher than saline group（P〈0.05）,but had no significant differences compared with Kangfuxin group（P〈0.05）.The healing time of GTX group was shorter than saline group（P〈0.05）,but had no significant differences compared with Kangfuxin group（P〈0.05）.Conclusion Guangtongxiao foam aerosol can relieve pain of the mice and promote wound healing on the rats measurably.The higher dose is used,the better efficacy will be obtained.