PARP-1基因多态性与临夏回族自治州回族人群胃癌易感性的关联研究

Correlation Between the Nucleotide Polymorphisms of PARP-1 and the Susceptibility of Gastric Cancer in Hui Ethnic Group of Linxia Hui Autonomous Prefecture

目的探讨聚腺苷二磷酸核糖聚合酶-1(PARP-1)Val762Ala的单核苷酸多态与临夏回族自治州回族人群胃癌易感性的关系。方法采用病例-对照研究设计,研究对象为临夏回族自治州居民(20岁以上,居住满20年以上,3代无族外通婚)。分两组:胃癌组200例,均经组织学确诊。对照组210例,按年龄、性别进行匹配的无肿瘤史的健康回族志愿者。抽提血液DNA,采用聚合酶链反应-限制性片段长度多态性方法(PCR-RFLP)进行基因分型。使用ELISA(enzyme-linkedimmunosorbent assay)法检测血浆中H.pylori的IgG抗体,以检测研究对象是否有H.pylori感染。用SPSS 13.0统计软件分析试验结果。结果 PARP-1 762Val/Ala+Ala/Ala在胃癌组显著增高(OR:2.158,95%CI:1.404～3.315,P=0.020)。交互作用分析显示:家族史阳性的Val/Ala+Ala/Ala基因型患胃癌的风险是家族史阴性的Val/Val基因型的4.125倍(OR:4.125,95%CI:2.117～8.038,P=0.000);食腌菜的Val/Ala+Ala/Ala基因型患胃癌的风险是不食腌菜的Val/Val基因型的3.814倍(OR:3.814,95%CI:2.150～6.766,P=0.000);H.pylori感染阳性的Val/Ala+Ala/Ala基因型患胃癌的风险是H.pylori感染阴性Val/Val基因型的4.085倍(OR:4.085,95%CI:2.197～7.597,P=0.000)。结论 PARP-1 Val762Ala单核苷酸多态与临夏回族自治州回族人群胃癌易感性增高相关。PARP-1 Val/Ala+Ala/Ala基因型分别与家族史、食腌菜、H.pylori感染在胃癌的发病风险中存在着加乘交互效应。

Objective To explore the correlation between nueleotide polymorphisms in polymerase - 1 （ PARP - 1 ） Va1762Ala and the susceptibility of gastric cancer in Hui ethnic group of Linxia Hui Autonomous Prefecture. Methods A case - control study was per- formed. All investigated objects were Hui ethnic population of Linxia Hui Autonomous Prefecture that included 2 groups： gastric cancer group （200 gastric cancer patients） , and control group （210 controls, frequency - matched for age and sex）. Polymerase chain reaction - restrieted fragments length polymorphism （PCR - RFLP） method was used to detect genotypes of PARP - 1Va1762Ala polymorphisms. ELASA（ enzyme - linked immunosorbent assay） method was used to identify IgG to H. pylori. The results were analyzed by SPSS13.0. Results PARP - 1762Val/Ala ＋ Ala/Ala genotypes were overrepresented in the gastric cancer group （ OR： 2. 158,95% CI ： 1. 404 - 3. 315 ,P = 0. 020）. Interaction analysis showed that： Val/Ala ＋ Ala/Ala genotypes carriers with positive family history of gastric cancer had 4. 125 fold increased risk for developing gastric cancer compared with Val/Val genotypes carriers with negative family history of gas- tric cancer （ OR ：4. 125,95 % C I ： 2. 117 - 8. 038, P = O. 000） ; Val/Ala ＋ Ala /Ala genotypes carriers with eating pickle vegetables had 3. 814 fold increased risk for developing gastric cancer compared with Val/Val genotypes carriers with not- eating pickle vegetables（ OR： 3. 814,95% CI：2. 150- 6. 766 ,P = O. 000）. Val/Ala ＋ Ala/Ala genotypes carriers with positive H. pylori infection had 4. 085 fold increased risk for developing gastric cancer compared with Val/Val genotypes carriers with negative H. pylori infection（ OR ：4. 085,95% CI： 2. 197 - 7. 597,P = 0. 000）. Conclusion PARP - 1Va1762Ala contributed to the increased suseeptibility of gastric cancer in Hui ethnic group in Linxia Hui Autonomous Prefecture. Muhiplicative interactions lied in between PARP - 1762Val/Ala ＋ Ala/Ala genotypes and positive family history of gastric cancer, eating pickle vegetables, H. pylori infection in risk of developing gastric cancer.