LTR对J亚群禽白血病病毒感染的影响

Effect of LTR on avian leukosis virus subgroup J infection

为探讨LTR基因在骨髓瘤病变型J亚群禽白血病病毒(ALV-J)NX0101致病中的作用,利用反向遗传将血管瘤病变型ALV-J HN06株中两端LTR元件替换至NX0101株的相应位置,拯救出重组病毒NX-HNLTR株。人工接种7日龄SPF雏鸡,分别检测NX0101株和NX-HNLTR株对鸡体的影响。感染鸡生长都较慢。感染NX0101株的鸡,胸腺指数和腔上囊指数明显比对照组低,脾脏指数与对照组相比波动较大,骨髓和脾脏在攻毒后3周可检测到病毒整合到基因组中,胸腺和腔上囊在攻毒后6周才检测到。感染NX-HNLTR株的鸡脾脏指数明显比对照组低,攻毒后2周可检测到病毒整合到脾脏基因组中,骨髓和胸腺分别在攻毒后3周和6周检测到。结果提示,LTR对NX0101株感染鸡的免疫器官有一定的影响。

In order to rescue the LTR element-substitution virus,the recombinant plasmid pNX-HNLTR containing the whole NX0101 genome with LTR element of hemangiomas strain HN06 was constructed.The plasmid was transfected into DF-1 cells and virus NX-HNLTR was rescued.To analog early infection,SPF birds were inoculated with strain NX0101 and NX-HNLTR at 7 day-old,respectively.Results showed that NX0101 suppressed significantly body weight,thymus and bursa,while NX-HNLTR suppressed body weight and spleen.Viral integration of NX0101 were firstly detected in the genome of bone marrow and spleen at 3 week post viral infection（wpi）,and then bursa and thymus at 6 wpi.While viral integration of NX-HNLTR were firstly detected in the genome of spleen at 2 wpi,and then bone marrow and thymus at 3 wpi and 6 wpi,respectively.The results showed that LTR element probably plays an important role in ALV-J NX0101 infection in immune organs.