人类杯状病毒医院感染调查研究

Investigation and research on nosocomial infections caused by human calicivirus

目的调查研究广州地区人类杯状病毒(HuCV)的感染流行特征。方法收集广东省中医院病毒性腹泻粪便标本380份,用荧光定量PCR方法检测样本中的HuCV[包括诺瓦克样病毒(NLVs)和扎幌样病毒(SLVs)]核酸。结果人类杯状病毒总检出率为28.68%,其中NLVs和SLVs检出率分别为22.89%、7.11%;HuCV在4个季度的感染率分别为46.29%、10.09%、6.42%和36.70%;另外,1例样本同时感染了GⅡ型NLVs和GⅡ型SLVs,有4例样本同时感染了GⅠ、GⅡ型NLVs。结论 HuCV是导致广州地区病毒性腹泻的重要病原体之一,高发期集中在第一、四季度,诺瓦克样病毒在HuCV感染中占主导作用;广州地区存在着HuCV不同基因型的混合感染。

OBJECTIVE To investigal：e the epidemic status of human calicivirus （HuCV）：infections in Guangzhou. METHODS A total of 380 cases of viral fecal specimens with gastroenteritis from Traditional Chinese Medicine Hospital of Guangdong were collected; fluorescence quantitative PCR method was adopted to detect the nucleic acid of HuCV （including NLVs and SLVs）. RESULTS The total positive rate of HuCV was 28.68%, the positive rates of NLVs and SLVs were 22.89%and 7.11%, respectively; the rates of HuCV infections in four seasons were 46.29%, 10.09%, 6.42%, a：ad 36.70% respectively; there was one case infected with both GII NLVs and SLVs, 4 cases infected with both G Ⅰ and GⅡ NLVs. CONCLUSION HuCV is one of the major pathogens causing diarrhea in Guangzhou, the peak of HuCV infection occurs in the first and the fourth season; NLVs infection is the predominant HuCV infections; there are mixed infections： of various genotypes of HuCV in Guangzhou.