不同茶树品种中黄酮苷含量的测定

Determination of the Flavone Glycosides in Various Tea Cultivars

本研究采用液相质谱技术分离、鉴定了茶叶中的12种黄酮苷类化合物,包括1种芹菜素苷、2种杨梅素苷、5种槲皮素苷和4种山奈酚苷。结合标准品及高效液相色谱系统,测定了8种不同茶树品种中黄酮苷类化合物的含量。结果显示,不同茶树品种中黄酮苷含量分布广泛,以槲皮苷为主。紫芽品种中12种黄酮苷含量最高,达6.12 mg/g;福建水仙品种中含量最低,仅2.31 mg/g,茶树品种间存在显著性差异(P<0.05)。

A standardized profiling method based on high performance liquid chromatography combined with mass spectrometric detection （MS） was established to identify and quantify twelve flavone glycosides in eight tea cultivar. The result showed that the contents of flavonoid glycoside in different tea cultivars were widely distributed The content of flavonoid glycoside was highest in the tea cultivar of Ziya up to 6.12 mg/g, and lowest in the tea cultivar of Fujian Shuixian with only 2.31 mg/g. There was a significant difference between these two tea cultivars （P〈0.05）.