Identification of Phlebotomus chinensis(Diptera:Psychodidae) inferred by morphological characters and molecular markers

基于形态和分子特征的中华白蛉分类研究(双翅目:毛蠓科)(英文)

To clarify the taxonomic status of Phlebotomus chinensb, the morphological characters of Ph. chinensis from Shaanxi, Henan, Sichuan and Gansu Provinces, and Ph. wui and Ph. longiductus from Xinjiang were examined and the rDNA-ITS2 region and mtDNA Cyt b gene sequences were analyzed simultaneously. It was found that morphological variation was very low in Ph. chinensis among the different localities; the wings of adult samples distributed in high-altitude areas were merely longer. The rDNA-ITS2 and mtDNA Cyt b sequences had an intraspecific variability. The relationships among species in the genus Phlebotomus can be inferred by molecular markers. These results suggest that there is an obvious genetic divergence at the molecular level among the Ph. chinensis populations in China.

为准确鉴别中华白蛉,探讨其分类地位,对采自陕西、河南、四川和甘肃的中华白蛉,以及新疆的吴氏白蛉、长管白蛉进行了形态学观察和分子特征分析。检视了成虫的形态鉴别特征,测定和分析了其核糖体DNA第2内转录间隔区(rDNA-ITS2)和线粒体DNA细胞色素b(mtDNA-cyt b)基因部分序列。结果显示各地中华白蛉形态变异小,高海拔地区的个体仅翅较长。ITS2和Cyt b序列在中华白蛉种内均具异质性,但其间的差异程度能较好地解析白蛉属部分种间的关系。本研究结果提示中华白蛉种群在形态和分子水平已出现明显遗传分化。