ARK5、MMP-2和MMP-9在胃癌中的表达及与侵袭转移的关系

The expressions of ARK5,MMP-2,and MMP-9 in gastric cancer and their relationships with the metastasis of gastric cancer

目的:探讨ARK5、基质金属蛋白酶2(matrix metalloproteinase-2,MMP-2)、基质金属蛋白酶9(matrix metalloproteinase-9,MMP-9)在胃癌组织中的表达及与胃癌侵袭转移的关系。方法:免疫组织化学方法检测66例胃癌组织及20例正常胃组织中ARK5、MMP-2、MMP-9的表达;脂质体介导法将ARK5-siRNA转染入胃癌细胞株SGC-7901(siARK5/SGC7901细胞组),以SCRsiRNA转染组(scr/SGC7901细胞组)作为对照组,Western blot检测转染后ARK5、MMP-2、MMP-9蛋白表达;Transwell侵袭实验检测细胞体外侵袭能力。结果:ARK5、MMP-2、MMP-9在胃癌组织中的阳性表达率分别为68%、76%、68%,三者之间差异具有统计学意义(P<0.05);ARK5的表达与胃癌的浸润深度、细胞分化程度、TNM分期及淋巴结转移呈正相关(P<0.05),与年龄、性别、肿瘤大小无关(P>0.05);转染后siARK5/SGC7901细胞组表达ARK5、MMP-2和MMP-9蛋白水平降低,体外侵袭能力明显降低(P<0.05)。结论:ARK5、MMP-2和MMP-9在组织中的表达有相关性,ARK5与胃癌的侵袭和转移密切相关,可作为判断预后的指标和化学治疗的靶点。

Objective To investigate the expressions of ARK5,MMP-2,and MMP-9 in gastric cancer and their relationships with the invasion and metastasis of gastric cancer.Methods The expressions of ARK5,MMP-2,and MMP-9 in 66 cases of gastric cancer tissues and 20 cases of normal gastric tissues were measured by immunocytochemistry;.ARK5-siRNA was transfected into SGC-7901 cell lines by liposome mediated-method（siARK5/SGC7901 group）,.with SGC-7901 cell lines transfected by SCRsiRNA as control group..The expressions of ARK5,MMP-2,.and MMP-9 were measured by Western blotting analysis.The in vitro invasion ability was examined by Transwell invasion assay.Results The expression rates of ARK5,MMP-2,and MMP-9 in gastric cancer tissues were 68%,76%,and 68%,respectively,and there were significant differences among them（P0.05）.The expression of ARK5 was positively correlated with the depth of invasion,the degree of cellular differentiation,the TNM staging,and the lymph node metastasis（P0.05）,but was not related to the age,sex,and tumor diameter（P0.05）.The protein levels of ARK5,MMP-2,and MMP-9 in siARK5/SGC7901 group were obviously decreased and the in vitro invasion ability were significant decreased（P0.05）.Conclusions There were significant correlations among the expressions of ARK5,MMP-2,and MMP-9.The expression of ARK was closely correlated with the invasion and metastasis of gastric cancer,wich can be used as a prognosis index and chemical treatment targets for gastric cancer.