IGF-I、IGF-II及其受体在肝癌和癌旁肝组织中的表达

Expressions of IGF-I, IGF-II and their receptor mRNAs in hepatocellular carcinomas and adjacent non tumor tissues

目的 :探讨胰岛素样生长因子I、II及其受体在肝癌和癌旁肝组织中的表达及其意义。方法 :采用DNA RNA原位杂交方法检测肝癌和癌旁肝组织中IGF I、IGF II及其受体mRNA的表达。结果 :在肝癌组织中IGF I、IGF I受体、IGF II、IGF II受体mRNA的表达率分别为 4 0 .0 %、4 6.7%、66.7%和 63 .3 % ,在癌旁肝组织中IGF I、IGF I受体、IGF II、IGF II受体mRNA的表达率分别为 4 6.7%、5 3 .3 %、70 .0 %和 73 .3 % ,有 3 6.7%的肝癌和 5 0 .0 %的癌旁肝组织同时表达四种mRNA。IGF I、IGF II及其受体mRNA在分化较差的肝癌细胞、肝细胞再生结节和不典型增生肝细胞中阳性信号最强。结论 :IGF I、IGF II及其受体在肝细胞癌变过程中发挥重要作用 ,肝癌的发生发展与IGF I、IGF IR、IGF II、IGF

Objective: To clarify the clinical significance and effect of the expression of IGF I,IGF II and their receptors in hepatocellular carcinomas (HCC) and adjacent non tumor tissues. Method: In situ hybridization was used to detect the mRNAs for IGF I,IGF II and their receptors in HCC and adjacent non tumor tissues. Results:The expressions of IGF I, IGF IR, IGF II, and IGF IIR mRNA were detected in 40.0%, 46.7%, 66.7%and 63.3%, respectirely in HCC. Positive hybridization signals of IGF I, IGF IR, IGF II, and IGF IIR mRNA were found in 46.7%, 53.3%, 70.0% , and 73.3%of adjacent non tumor tissues.The liver cell dysplasia and poorly differentiated HCC cells were stained strongly positive. Conclusion:Coordinate overexpression of IGF I, IGF II and their receptors might play an important role in the formation and development of HCC.