期刊文献+

环孢素A对体外人系膜细胞cyclin E,cyclin D1,p27^(kip1)蛋白表达的影响 被引量:2

Effect of CsA on protein expression of cyclin D1,cyclin E and p27^(kip1) in cultured human mesangial cells in vitro
下载PDF
导出
摘要 目的探讨环孢素A(CsA)对人系膜细胞增殖的抑制作用及细胞周期蛋白D1、E、p27kip1表达影响。方法人系膜细胞常规体外培养,分为实验组和对照组,实验组加入不同浓度的CsA(0.1,1,5μmol/L)干预。药物作用24,48,72 h后MTT比色法测定细胞增殖情况。药物作用48 h后采用流式细胞仪检测细胞周期。药物作用48 h后Western blot法检测细胞中cyc-lin D1、cyclin E、p27kip1蛋白质表达情况。结果 CsA对人系膜细胞具有时间、浓度依赖性抑制作用;且浓度依赖性阻滞细胞周期,使G0/G1期细胞增多,S期细胞减少(P<0.05)。CsA浓度依赖性下调cyclin D1、cyclin E蛋白,上调p27kip1蛋白的表达(P<0.05)。结论 CsA通过下调cyclin D1、cyclin E蛋白,上调p27kip1蛋白的表达,调节细胞周期调控通路,阻滞细胞于G0/G1期,有效抑制人系膜细胞的增殖。 Objective To explore the effect of CsA on proliferation,apoptosis and cell cycle of human mesangial cells. Methods Human mesangial cells were incubated in vitro with different concentrations of CsA(0,0.1,1,5 μmol/L).The proliferation of human mesangial cells was detected after incubated with different concentrations of CsA for 24,48,72 h by MTT assay,respectively.Cell cycle was analyzed at 48 h after incubation by flow cytometry.The expression of cyclin D1,cyclin E and p27kip1 proteins in cell nucleus was assessed by Western blot. Results The proliferation of human mesangial cells was inhibited in a time-and dose-dependent manner by CsA.The cell cycle was arrested in G0/G1 phase.The percentage of G0/G1 phase cells gradually increased with the rising of CsA concentrations(P0.05).The expression of cyclin D1,cyclin E proteins decreased in cell nucleus with the rising concentrations of CsA,while expression of p27kip1 protein increased significantly(P0.05). Conclusion CsA could inhibit the mesangial cell proliferation by decreasing the expression of cyclin D1,cyclin E proteins and increasing the expression of p27kip1 protein.
出处 《山西医科大学学报》 CAS 2012年第4期245-248,共4页 Journal of Shanxi Medical University
关键词 环孢素A 人系膜细胞 CYCLIN D1蛋白 CYCLIN E蛋白 P27KIP1蛋白 增殖 cyclosporine A human mesangial cell cyclin D1 protein cyclin E protein p27kip1 protein proliferation
  • 相关文献

参考文献5

二级参考文献54

  • 1戴振华,李晓玫,王海燕.白细胞介素-10对大鼠肾小球系膜细胞炎症效应的拮抗作用[J].中华医学杂志,1996,76(6):407-410. 被引量:14
  • 2Chan H L,Hui A Y,Wong M L,et ai. Genotype C hepatitis B virus infection is associated with an increased risk of hepatocelhlar carcinoma[J]. Gut,2004,53(10) :1494-1498.
  • 3Verkhivker C M. Protein conformational transitions coupled to binding in molecular recognition of unstructured proteinsl hierarchy of structural loss from all-atom Monte Carlo simulations of p27Kipl unfolding-unbinding and structural determinants of the binding mechanism [J]. Biopolymers, 2004, 75 (5) :420-433.
  • 4Zhang W, Tong Q, Wu Q, et al. Upregulated p27kip1 can downregulate survivin expression and inhibit telomerase activity in gastric carcinoma cel[J]. Cancer Invest, 2009,27 (9): 898-900.
  • 5Hong YB, Kang HJ, et al. Inhibition of cell proliferation by a resveratrol analog in human pancreatic and breast cancer cells [J]. Exp MolMed, 2009,41:151-160.
  • 6Verkhivker C M. Protein conformational transitions coupled to binding in molecular recognition of unstructured proteins: deciphering the effect of intermolecular interactions on computational structure prediction of the p27Kipl protein bound to the cyelin A-cyclin-dependent kinase 2 complex[J]. Proteins, 2005, 58(3) :706-716.
  • 7Bagui T K,Cui D, Roy S, et al. Inhibition of p27KIP1 gene transcription by mitogens[J]. Cell Cycle, 2009, 8 : 115-124.
  • 8Trabosh V A, Divito K A, D Aguda B, et al. Sequestration of E12/E47 and suppression of p27KIP1 play a role in Id2-indueed proliferation and tumorigenesis [J]. Caroinogenesis, 2009,30: 1252-1259.
  • 9Morishita D, Katayama R, Sekimizu K, et al. Pim kinases promote cell cycle progression by phosphorylating and down-regulating p27Kipl at the transcriptional and posttranscriptional levels[J]. Cancer Res, 2008,68 : 5076-5085.
  • 10Tossidou I,Dangers M, Koch A, et al. Tyrosine phosphatase SHP-2 is a regulator of p27 (KIP1)tyrosine phosphorylation [J].Cell Cycle, 2008, 7:3858-3868.

共引文献29

同被引文献7

引证文献2

二级引证文献3

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部