西比灵逆转抗癫痫药诱导的多药耐受基因表达

AEDs-induced Overexpression of Multidrug Resistance Gene Was Reversed by Sibelium

目的 研究西比灵逆转抗癫痫药（ＡＥＤｓ）诱导的星形胶质细胞多药耐受基因（ＭＤＲ１）表达的规律。方法 将苯巴比妥钠（ＰＢ）和丙戊酸钠（ＶＰＡ）分别加入培养的星形胶质细胞内，３０天后用免疫细胞化学法检测ＭＤＲ１的表达产物Ｐ－糖蛋白（Ｐｇｐ），继之将终浓度为５μｇ／ｍｌ的西比灵分别加入培养的正常细胞（亲本细胞）及ＭＤＲ１表达增强的细胞（耐药细胞）内，用四甲基偶氮唑盐（ＭＴＴ）法计算逆转倍数和相对逆转效率，了解西比灵逆转ＭＤＲ１表达的作用。结果 发现西比灵对耐ＰＢ或ＶＰＡ的星形胶质细胞的逆转数分别为２．０９、２．５１，相对逆转效率分别为７６．２５％、６７．８０％。结论 西比灵对ＡＥＤｓ诱导的ＭＤＲ１的表达有部分逆转作用。

Objective:To study the Sibelium(Flunarizine) reverses antiepileptic drugs(AEDs)- induced overexpression of multidrug resistance gene (MDR1). Methods: Phenobarbital and Valproic acid were added into astrocytes and cultured permanently for 30 days. P-glycoprotein (Pgp); a product of MDR1, was detected with immunocyto chemistry.Then,5μg/ml Sibelium was added to resistance cells(Pgp positive cells) and nonresistance cells(umstimulated by AEDs),MTT assay was used to measure reverse multiple and relative reverse efficiency rate.Results:Our study showed reverse multiples were 2~2. 5, and relative reverse efficiency rates were 68% ~76% by Sibelium. Conclusion: Sibelium may re-verse the overexpression of MDR1.