西比灵对大鼠急性脑缺血再灌注损伤自由基影响的实验研究

The effects of sibelium on the free radicals after acute cerebral ischemia reperfusion injury of the rats

目的 进一步探讨西比灵对脑缺血再灌注损伤保护作用的机制。方法采用Ｐｕｌｓｉｎｅｌｌｉ四血管闭塞法（４ＶＯ）制备大鼠急性 脑缺血再灌注模型，利用Ｔｓｕｃｈｉｄａ的ＷＳＰＳ法测定ＬＰＯ，利用化学发光法测定ＳＯＤ活性，并观察西比灵对上述指标的影响。结果西比 灵可降低ＬＰＯ含量，稳定ＳＯＤ活性。结论西比灵可抑制脑缺血再灌注时脑组织内自由基的生成。

Objective To study the protective effect of sibelium to acute cerebral ischemia reperfusion injury. Mathods Acute cerebral ischemia reperfusion rat model were prepared by pulsinelli's 4VO method. to assay LPO,Tsuchida's WSPS method,was used and SOD activity was assayed by chemiluminescence method to observe sibelium's influence on these indices. Results Sibelium can decrease LPO content,stabilize SOD activity and sibelium can also inhibit free radical production during cerebral ischemia reper fusion. Conclusions Sibelium can protect brain tissue during reper fusion after cerebral ischemia.