威灵仙的化学成分研究

Chemical Constituents of Clematis chinensisOsbeck

威灵仙是一种重要的中药材。运用快速硅胶柱层析和制备型HPLC技术对威灵仙的乙酸乙酯提取物进行分离,纯化得到7个化合物,它们是松脂素(Pinoresinol,1),Epipinoresinol(2),罗汉松脂素(Matairesinol,3),Salicifoliol(4),3,4,5-三羟基苯甲酸(5),4-羟基-3,5-二甲氧基-苯甲酸(6),异阿魏酸(7),化合物的结构通过NMR和MS的分析得到确定。运用分析型HPLC和GC-MS色谱分析方法,可以在威灵仙药材提取物中快速、准确地鉴定该类化合物。化合物1,2,3,4是木脂素类化合物,具有多种重要的生理活性,潜在药用明显。

Clematis chinensis Osbeck is a popular medicinal plant. Seven known compounds, pinoresinol （1）, epipinoresinol （2）, matairesinol （3）, salicifoliol （4）, 3, 4, 5-trihydroxybenzoic acid （5）, 4-hydroxy-3, 5-dimethoxybenzoic acid （6）, and 3-hydroxy- 4- methoxycinnamic acid （7） were isolated from the ethyl acetate extract of Clematis chinensis Osbeck by using the flash silica gel column chromatography and preparative high performance liquid chromatography. Their structures were elucidated mainly on the basis of NMR and MS data. These compounds could be fast and accurately detected from the extract by using HPLC and GCMS analytical methods. Lignans 1, 2, 3, 4 have various bioactivities, and the lignans from Clematis chinensis Osbeck should have the potential application in drug development.