逆灸命门穴对正常大鼠力竭游泳时间及HPA轴的影响

Effect of preventive moxibustion at Mingmen(DU-4) on swimming exhausted time and HPA axis hormone of rats

目的:观察逆灸命门穴对大鼠力竭游泳时间及游泳力竭后下丘脑—垂体—肾上腺(HPA)轴的影响。方法:将大鼠按游泳能力随机分为空白对照组、逆灸命门组、力竭对照组、逆灸命门+力竭组,分别干预20d后进行力竭游泳,观察力竭时间的差别,并采用生化法检测下丘脑促肾上腺皮质激素释放激素(CRH)、垂体促肾上腺皮质激素(ACTH)、肾上腺皮质醇(CORT)的含量。结果:逆灸命门+力竭组力竭游泳时间较力竭对照组明显延长(P<0.05)。各组内HPA轴的3种激素表现同步:与空白对照组相比,逆灸命门组大鼠的下丘脑CRH、垂体ACTH、肾上腺CORT含量无明显变化;力竭对照组大鼠的下丘脑CRH、垂体ACTH、肾上腺CORT含量极显著升高(P<0.01);与力竭对照组比较,逆灸命门+力竭组大鼠的下丘脑CRH、垂体ACTH、肾上腺CORT含量明显降低(P<0.01)。结论:运动力竭后HPA轴出现过度应激,逆灸命门穴可缓和其应激程度,这可能是逆灸命门穴能提高大鼠的运动耐力,延长其游泳力竭时间的部分机制。

Objective： To observe the effect of preventive acupuncture at Mingmen（DU-4） on exercise tolerance and HPA axis hormone of rats. Methods： Randomly divided rats into normal control group, Mingmen moxibustion group, exhaustion control group, Mingmen moxibustion ＋ exhaustion group according to their swimming exhausted time. Rats received a exhaustive swimming after 20 days of intervention. Exhausted time of swimming rats were recorded. The hypothalamus CRH, pituitary ACTH, adrenal gland CORT of rats were detected by biochemical method and compared. Results： The swimming exhausted time of Mingmen moxibustion ＋ exhaustion group were significantly increased compare to exhausted control group （P〈0.05）. Three types of hormone of HPA axis in each group have the same trend： Compared with normal control group, the hypothalamus CRH, pituitary ACTH, adrenal gland CORT of Mingmen moxibustion group had no significant differences, The HPA axis hormone of exhausted control group was significantly increased （P〈0.01）. The HPA axis hormone of Mingmen moxibustion ＋ exhaustion group were significantly lower than that of the exhausted control group（P〈0.01）. Conclusions： Preventive moxibustion at Mingmen（DU-4） could improve the exercise tolerance of rats and prolong their swimming exhausted time. Its physiological mechanism might be related to easing excessive stress of HPA axis on exhaustive exercise.