CD3^+、CD4^+、CD8^+T细胞水平在胃癌患者外周血中的变化

Change on CD3^+,CD4^+ and CD8^+ T lymphocyte levels in peripheral blood of patients with gastric cancer

目的探讨T淋巴细胞亚群CD3+、CD4+、CD8+和CD4+/CD8+比值水平在胃癌患者外周血中的表达及意义。方法采用血液分析仪技术,检测40例胃癌手术前患者(按临床分期为Ⅰ～Ⅱ级组18例,Ⅲ～Ⅳ级组22例)及25名正常对照组外周血CD3+、CD4+、CD8+细胞和CD4+/CD8+比值T细胞水平。结果胃癌患者外周血CD3+、CD4+细胞、CD4+/CD8+比值分别为0.78%±0.35%、0.37%±0.21%、0.61%±0.42%,明显低于正常对照组(P<0.05);胃癌Ⅲ～Ⅳ级组CD3+、CD4+细胞CD4+/CD8+比值分别为0.42%±0.27%、0.21%±0.17%、0.29%±0.25%,明显低于Ⅰ～Ⅱ级组(0.95%±0.35%、0.45%±0.19%、0.76%±0.30%,P<0.05)。结论检测胃癌患者手术前外周血CD3+、CD4+、CD8+细胞和CD4+/CD8+比值T细胞水平,可以初步评估胃癌患者机体免疫状态,也为肿瘤的免疫增强治疗提供了理论依据。

Objective To investigate the clinical significance and expression of peripheral blood T lymphocyte subset CD3^＋ , CD4^＋ , CD8^＋ and CD4^＋/CD8^＋ ratio in patients with gastric cancer. Methods Peripheral blood T lymphocyte subset CD3^＋ , CD4^＋ , CD8^＋ and CIM^＋/CD8^＋ ratio were determined by automatic blood cell analyzer in 40 patients with gastric cancer [ according to the clinical stages： Ⅰ-Ⅱ stage group （18 cases） and Ⅲ-Ⅳ stage group （22 cases） ] and 25 healthy subjects. Results The peripheral blood T lymphocyte subset CD3^＋ , CIM^＋ and CIM^＋/CD8 ^＋ ratio in the gastric cancer group were 0.78% ＋-0.35% ,0.37% ＋-0.21% and 0.61% ＋0.42%, respectively, which were significantly lower than those in the healthy control group （ P 〈 0.05 ）. The T lymphocyte subset CD3^＋ , CIM^＋ and CIM^＋/CD8^＋ ratio in Ⅲ-Ⅳ stage group were 0.42% ± 0.27 %, 0.21%±0.17 % and 0.29% ±0.25 %, which were significantly lower than those in Ⅰ-Ⅱ] stage group（0.95% ±0.35% ,0.45%±0. 19% and 0.76% ±0.30% ,P 〈 0.05）. Conclusions The determinations of T lymphocyte subset CD3^＋ , CIM^＋ , CD8^＋ and CD4^＋/CD8^＋ ratio in peripheral blood of patients with gastric cancer are helpful to study and assess the immune status of patients , but also provide a theoretical reference to improve tumor immune therapy .