鼠尾藻的化学成分研究

Chemical Constituents from Marine Alga Sargassum thunbergii

目的研究鼠尾藻的化学成分。方法采用正相及反相硅胶柱色谱、Sephadex LH-20凝胶柱色谱、半制备高效液相色谱等方法进行分离纯化,根据理化性质及波谱学数据鉴定化合物的结构。结果从鼠尾藻乙醇提取物中,分离鉴定了15个化合物,其中9个异戊二烯类化合物,分别为黑麦草内酯(1),异黑麦草内酯(2),loliolide acetate(3),蚱蜢酮(4),apo-9'-fucoxan-thinone(5),3α-hydroxy-5,6-epoxy-7-megastigmen-9-one(6),deacetylate-apo-13'-fucoxanthinone(7),apo-13'-fucoxanthinone(8),(R)-(-)-3-羟基-β-紫罗兰酮(9),6个其他类型的化合物,sargassum ketone(10),2-heptenoic acid-4,4-dihydroxy-2,3-dimethyl-γ-lactone(11),(R)-2-hydroxy-3-phenylpropanamide(12),脱氧核糖胸腺嘧啶(13),岩藻甾醇(14),甘油脂肪酸酯(15)。结论deacetylate-apo-13'-fucoxanthinone(7)为新化合物,化合物3,5,6,8,9,15为首次从该属植物中分离得到。

OBJECTIVE To study the chemical constituents of Sargassum thunbergii. METHODS The compounds were isola- ted and purified upon extensive column chromatography using silica gel, ODS, Sephadex LH -20, and prepative HPLC, while their struc- tures were identified on the basis of physicochemical properties and spectroscopic data analysis. RESULTS Fifteen compounds were isolated from the EtOH extract. The structures were identified as （ ＋ ）-isololiolide （1）, （ - ）-loliolide（2） ,loliolide acetate（3） ,grass- hopper ketone （4）,apo-9＇-fucoxan-thinone（5）,3α-hydroxy-5,6-epoxy-7-megastigmen-9-one（6）,deacetylate-apo-13＇-fucoxanthinone（7）,apo-13＇-fucoxanthinone（8）,（R）-（-）-3-hydroxy-β-ionone （ 9 ） and 6 other compounds sargassum ketone（10）,2-heptenoic acid-4,4-dihydroxy-2,3-dimethyl-γ-lactone（11）,（R）-2-hydroxy-3-phenylpropanamide（12）, thymidine （ 13 ）, fucosterol （ 14 ） and hexade- canoic acid-2＇ ,3＇-dihydroxypropyl ester（15）. CONCLUSION Deacetylate-apo-13＇-fucoxanthinone is a new compound, and com- pounds 3,5,6,8,9,15 are obtained from the genus Sargassum for the first time.