硫氰酸生成酶样基因在家蝇高温和重金属镉应激中的作用

Roles of Rhodanese-like Gene in Stress Responses of Musca domestica toward Hyperthermy and Cadmium Tolerance

硫氰酸生成酶同源结构域普遍存在于古细菌、真细菌以及真核生物的多种蛋白分子内,这些蛋白构成一个功能多样的硫氰酸生成酶超家族。本研究根据家蝇转录组序列信息,分拣到一条硫氰酸生成酶样基因的EST序列,经分析发现其编码蛋白C末端含有典型的硫氰酸生成酶同源结构域,属于硫氰酸生成酶超家族,将其命名为家蝇硫氰酸生成酶样基因(Md-Rhodlike)。应用实时定量RT-PCR技术,分析了Md-Rhodlike在热激、镉胁迫条件下的表达量变化。Md-Rhodlike的mRNA水平在高温和镉处理后上调,提示该基因可能参与家蝇对高温及镉金属等环境胁迫因子的耐受,是一种新的家蝇应激相关蛋白。

The rhodanese homology domain is a ubiquitous fold found in various proteins encoded by eubacterial, archeal, and eukaryotic genomes, which form the pluripotent rhodanese superfamily. In the light of the transcriptomic information of Musca domestica, a rhodanese-like gene EST was identified and referred to as Md-Rhodlike, which encodes a protein of rho- danese superfamily with a representative rhodanese homology domain at the C-terminal end. Expression profiles of Md-Rhod- like in response to heat shock and cadmium stress were detected via real-time quantitative RT-PCR. The mRNA level of Md- Rhodlike was upregulated after heat shock and Cd-exposure. These results indicated that Md-Rhodlike might be a noval stress-resistance-associated protein in Musca domestica, which plays an important role in hyperthermy and cadmium toler- ance.