病毒性心肌炎小鼠模型中Caveolin-3表达及银杏叶提取物的相关干预研究

The research on Caveolin-3 expression and intervention effect of Ginkgo biloba extract in mice model with viral myocarditis

目的观察小凹蛋白-3(Caveolin-3)在病毒性心肌炎(viral myocarditis,VMC)小鼠模型心肌组织中的表达情况及银杏叶提取物的干预效果。方法选取160只Balb/c小鼠,随机分成正常对照组、病毒性心肌炎组(VMC)、参麦治疗组(SM)、银杏叶提取物治疗组(GL),每组各40只。建立VMC小鼠模型。在柯萨奇病毒B3(CVB3)感染后第5、10、15天各组随机处死8只小鼠,HE染色、Masson染色法观察心肌病变,半定量RT-PCR方法检测小鼠心肌组织中Caveolin-3 mRNA的相对表达量,并作相关性分析。结果 VMC小鼠的心肌表现为炎性病变;SM及GL组小鼠的炎性改变在各时间点均较VMC组轻;VMC组小鼠第10、15天的CVF明显高于对照组(P均<0.01),SM及GL组小鼠均较VMC组改变轻(P均<0.01);VMC组小鼠Caveolin-3 mRNA的相对表达量随着时间的推移增加明显;SM组及GL组干预后,Caveolin-3 mRNA的相对表达量亦有所降低(P均<0.05)。Caveolin-3 mRNA的相对表达量与病理积分、CVF密切相关(r=0.959、-0.896,P均<0.05)。结论 Caveolin-3可能参与了病毒性心肌炎的发病,银杏叶提取物对病毒性心肌炎具有一定的治疗作用。

Objective To study the expression of Caveolin-3 in mice model with viral myocarditis （VMC） and the intervention effect of Ginkgo biloba extract. Methods One hundred and sixty Balb/c mice were selected and randomly divided into 4 groups： control group （n = 40）, VMC group （n = 40）, SM group （n = 40） and GL group （n = 40）. VMC models was established. On day 5, 10, 15 after CVB3 infection, 8 mice were randomly killed in each group. Patho- logical changes of cardiomyocytes were examined by HE staining and Masson stain. The relative level of Caveolin-3 mRNA expression was detected by semi-quantitative reverse transcription-polymerase chain reaction （SqRT-PCR） , and the correlations were analyzed. Results In VMC group, cardiomyocytes of mice had inflammatory lesions by HE staining. VMC mice in SM and GL group which received either SM or GL treatment had smaller inflammatory changes compared with VMC group at each time point. The CVF values of VMC group were significantly higher than that of control group on the 10 d and 15 d （P 〈 0.01 and P 〈 0.01, respectively）, and SM and GL group changed less than VMC model group on the 10 d and 15 d （P 〈 0.01 and P 〈 0.01, respectively）. The relative expression level of Caveolin-3 mRNA in VMC group at each time point increased significantly over time. After intervenedwith SM or GL, relative expression level of Caveolin-3 mRNA also decreased （P 〈 0.05 and P 〈 0.05, respectively） . The relative expression level of Caveolin-3 mRNA in myocardial tissue of VMC mice is closely correlated with pathological score and CVF （r = 0.959, P 〈 0.05 and r = -0.896, P 〈 0.05, respectively）. Conclusions Caveolin-3 may be involved in the process of myocarditis in mice. Ginkgo biloba extract has some therapeutic effect on viral myocarditis.