低温胁迫下24-表油菜素内酯对辣椒种子萌发及幼苗生长的影响

Effects of 24-epibrassinosteroids on the seed germination and seedling growth of pepper under low temperature stress

以华椒15号辣椒种子为材料,采用浸泡法和添加法2种方法,研究低温(15℃)胁迫下不同质量浓度24-表油菜素内酯(EBR)对辣椒种子萌发及幼苗生长的影响。结果表明:在0.001～1.000mg/L质量浓度范围内,EBR对辣椒种子发芽势没有显著影响;浸泡法中,0.001～0.100mg/L的EBR对辣椒种子的萌发率产生显著促进作用,0.050mg/L时促进作用最强,发芽率达44.00%;添加法中,在设定的质量浓度范围内,EBR对辣椒种子的萌发率产生抑制作用,1.000mg/L时抑制作用达差异显著水平。浸泡法和添加法中,0.001～1.000mg/L的EBR对辣椒幼苗的根长均产生显著抑制作用,1.000mg/L时抑制作用最强。浸泡法中,0.010～1.000mg/L的EBR对辣椒幼苗的苗高产生显著促进作用,0.050mg/L时促进作用最强,平均苗高为1.82cm,比对照高0.62cm;而添加法中,EBR对辣椒幼苗的苗高不产生显著影响。浸泡法中,0.001～1.000mg/L的EBR对辣椒幼苗的鲜质量和干质量不产生显著影响,而添加法中,EBR对辣椒幼苗鲜质量和干质量的影响没有规律性。

Effects of 24-epibrassinosteroids（EBR） on the seed germination and seedling growth of pepper（Huajiao 15） under the low temperature stress were investigated by both soaking method and ad- dition method. The results showed that 0. 001-1. 000 mg/L EBR had no significant effect on the germ- inability of pepper seed,0. 001-0. 100 mg/L EBR significantly promoted the germination rate of pepper seed with soaking method at 0. 050 mg/L with the germination rate of 44. 00%. The content of EBR ran- ging from 0. 001 mg/L to 1. 000 mg/L had inhibition on the germination rate of pepper seed under addi- tion method at 1. 000 mg/L. Under both soaking method and addition method, the content of EBR ran- ging from 0. 001 mg/L to 1. 000 mg/L significantly inhibited the seedling root length of pepper seed with the highest inhibition rates at 1. 000 mg/L, respectively. It also significantly promoted the seedling height of pepper seed under soaking method with 1.82 cm of seedling height, 0. 62 cm higher than that of control at the concentration of 0. 050 mg/L,but under addition method EBR had no significant effect on the seedling height of pepper seed. Under soaking method, the content of EBR ranging from 0. 001 mg/L to 1. 000 mg/L had no significant effect on green weight and dry weight of pepper seedling. But there was no regularity under addition method.