适于贵州玉米种质DNA指纹库构建的SSR核心引物筛选

Screening of SSR Core Primer Pairs Suitable for Establishing DNA Fingerprinting Pool of Guizhou Maize Cultivars

为了筛选出适用于贵州玉米种质DNA指纹库构建的引物,以21份贵州普通玉米自交系和36份糯玉米品种为材料,综合比较PIC值大小、扩增带型清晰度及引物的重复性高低,对87对SSR引物进行筛选,以确定贵州玉米种质DNA指纹库构建的核心SSR引物。结果表明:筛选出的23对和15对SSR引物可分别作为构建贵州普通玉米和贵州糯玉种质DNA指纹库的核心引物。

To screen SSR core primer pairs which were suitable for establishing DNA fingerprinting pool of Guizhou maize cultivars, 21 common maize inbred lines and 36 waxy maize cultivars as the materials were used to screen 87 sets of SSR primer pairs. Finally, 23 sets of core primer pairs fit for establishing maize DNA fingerprinting pool of Guizhou were confirmed with consideration of PIC value, the definition of amplified band pattern and the repeatability of primer. Moreover, 13 sets of core primer pairs fit for SSR molecular markers of Guizhou waxy corn were confirmed.