人巨细胞病毒AD169株UL115基因编码gL蛋白序列分析及B细胞表位预测

Study on B-cell epitopes of glycoprotein L expressed by human cytomegalovirus UL115 gene of AD169 strain

目的:分析人巨细胞病毒(HCMV)AD169株UL115基因序列,预测UL115基因编码的gL蛋白B细胞优势表位。方法:基于UL115基因编码的gL蛋白的氨基酸序列,结合亲水性参数、可及性参数、抗原性参数、柔韧性参数及二级结构方案对HCMVgL蛋白的B细胞表位进行预测,参照已建立的预测方法综合评价B细胞优势表位。结果:①UL115核酸变异集中在序列的N端,大部分是同义突变,氨基酸序列高度保守;②HC-MVgL蛋白B细胞表位可能位于编码蛋白N段197～205、253～261位。结论:多参数预测gL蛋白的B细胞优势表位,为进一步研究蛋白特征、制备单克隆抗体及表位疫苗提供依据。

Objective：To analyze the sequence of genome of cytomegalovirus UL115 and predict the B cell epitopes of the gL protein expressed by cytomegalovirus UL115.Method：The hydrophilicity,accessibility,antigenicity and flexibility index were used to predict the potential B cell epitopes of gL protein based on gL genome sequence.Result：① Most amino acid sequence of gL was highly conserved although several strains had variation.Those mutations focused on the N end of U115,but most of them were sense mutation.② The B cell epitopes of gL protein generated by combined application were predicted at gL protein N-terminal 197-205,253-261.Conclusion：The B-epitopes of gL protein was predicted successfully,which established the basis of the characterization of the protein,development of epitopes based vaccine,and preparation of monoclonal antibody against fusion protein.