脂蛋白脂肪酶-447C/G基因多态性与老年脑梗死的关系

The Relationship Between Lipoprotein Lipase-447C/G Genepolymorphism and Cerebral Infarction in the Elderly

目的探讨脂蛋白脂肪酶基因-447C/G多态性与我国老年脑梗死的关系。方法采用病例-对照研究,对206例老年脑梗死患者和203例非脑血管病患者(对照组)采用多聚酶链反应-限制性片长多态性对-447C/G基因多态性进行分析。结果脑梗死组中CC、CG+GG基因型和C、G等位基因的频率分别是83.98%、16.02%和91.75%、8.25%;对照组中CC、CG+GG基因型和等位基因C、G的频率分别是81.77%、18.23%和90.89%、9.11%。-447C/G基因型分布和等位基因频率在脑梗死组与对照组之间差异无显著性(P>0.05);各基因型之间血脂、血压水平差异无显著性(P>0.05)。脑梗死多因素回归方程的主要危险因素包括吸烟史、糖尿病史、家族史。结论 (1)-447C/G基因多态性可能与老年脑梗死的发病无关。(2)-447C/G基因多态性与老年人的血脂及血压可能无关。

Aim To explore the relationship between the lipoprotein lipase （LPL） -447C/G gene polymor- phism and cerebral infarction in the elderly. Methods This was a case-control study, which enrolled 206 cases with cerebral infarction in the elderly and 203 elderly incerebrovascular disease controls. The genotype and allele frequencies of lipoprotein lipase - 447C/G gene polymorphism were assayed by polymerase chain reaction-restricted fragment length pol- ymorphism （PCR-RFLP）. The cerebral infarction patients were strictly diagnosed according to revise conference in the national fourth session of cerebrovascular disease. The basic document object of study was collection through questionnaire interview. Results The LPL -447C/G genotype and allele distribution in the cerebral infarction group was CC 83.98%, CG ＋ GG 16.02% and C 91.75%, G 8.25% respectively. The distribution in control group was CC 81.77% , CG ＋ GG 18. 23% and C 90. 89% , G 9. 11% respectively. There was no significant difference in LPL - 447C/G allele and genotype distribution between the cerebral infarction group and the control group （P 〉 0. 05 ）. There was no significant difference detected in blood pressure level and blood lipids level between various genotypes （P 〉 0. 05 ）. Cerebral infarction multi-factor regression equation indicated primary hazard factors included smoking history, diabetes his- tory and family history. Conclusions （1）LPL-447C/G gene polymorphism is not significantly associated with cere- bral infarction in elderly. （2） LPL -447C/G gene polymorphism is not significantly associated with blood lipids level, blood pressure level in elderly.