杂交鲿(乌苏里拟鲿♀×瓦氏黄颡鱼♂)胚胎发育的研究

Studies on Embryonic Development of Crossbreed F1 by Pseudobagras Ussuriensis(♀)×Pelteobagrus Vachelli(♂)

采用LRH-A2+HCG+DOM混合催产剂对乌苏里拟鲿及瓦氏黄颡鱼进行催产.采取半干法受精的方式进行乌苏里拟鲿、瓦氏黄颡自交及乌苏里拟鲿(♀)×瓦氏黄颡鱼(♂)组合的人工授精,孵化水温为24℃～27℃,在解剖镜下进行胚胎发育观察,描述胚胎发育时序和形态特征.结果获得了23个时期的胚胎发育照片;乌苏里拟鲿、瓦氏黄颡鱼自交受精卵分别经过50 h 9 min、46 h 50 min孵化出膜;杂交鲿受精后28 min胚盘形成,受精后1 h进入卵裂期,4 h 23 min进入囊胚期,11 h 3 min进入原肠胚阶段,受精后16 h 56 min胚孔封闭,当受精38h 53 min后心脏开始出现搏动,受精后49 h 37 min仔鱼开始孵出.杂交鲿F1的胚胎发育时间介于两亲本之间,杂交苗全长偏向母本乌苏里拟鲿.

The mixture of LRH - A2 ＋ HCG ＋ DOM was injected into Pseudobagras ussuriensis and Pelteobagrus vachelli, before their embryonic development was observed by Nikon 80 iopticalm icroscope. The results showed that： at 24℃ - 27℃ of water temperature, 23 fetation photographs were taken to describe the whole process. The hatching time of Pseudobagras ussuriensis and Pelteobagrus vachelli were 50 h 9 min and 46 h 50 min respectively. The blastodisc of cross- breed Flformed at 28 min after fertilization, cleavage began at 1 h and blastula stage appeared at 4 h 23 min, gastrula stage at 11 h 3 min. Blastopore closed at 16 h 56 min and heart began beating rhythmically at 38 h 53 min. Some embryos began to break the membrane and came out at 49 h 37 min after fertilization. The total embryonic development time of crossbreed F1 was between that of Pseudobagras ussuriensis and Pelteobagrus vachelli. The total length of crossbreed F1 was more similar to that of the female parent.