GC-MS和HELP法分析药对陈皮-青皮与其单味药挥发油成分

Analysis of Volatile Constituents in Herbal Pair Pericarpium Citri ReticulataePericarpium Citri Reticulatae Viride and Its Single Herbs by GC-MS and HELP Method

采用水蒸气蒸馏法提取药对陈皮-青皮与其单味药的挥发油成分,利用气相色谱-质谱法(GC-MS)和化学计量学多元分辨方法——直观推导式演进特征投影法(HELP),同时结合程序升温保留指数(PTRI)进行挥发油成分定性定量分析。分别在陈皮、青皮和药对陈皮-青皮的挥发油中鉴定出27、27和23个成分,定性组分含量分别占三者挥发油总含量的93.64%、96.45%和85.28%,共有化合物19种;主要化学成分为D-柠檬烯、γ-萜品烯、芳樟醇和α-法呢烯。

The essential oil components in herbal pair（HP） Pericarpium Citri Reticulatae- Pericarpium Citri Reticulatae Viride（ PCR- PCRV） and its single herbs were respectively extracted by steam distillation, then qualitatively and quantitatively analyzed by gas chromatography -mass spectrometry （GC -MS ） and a chemometric resolution method, namely heuristic evolving latent projections （HELP）. In addition, temperature-programmed retention indices（PTRIs） were employed to improve the precise degree of qualitative analysis. To sum up, 27, 27 and 23 volatile chemical components in essential oils of PCR, PCRV and HP PCR -PCRV were separated and identified, accounting for 93.64% , 96. 45% and 85.28% of total contents of the three tested samples, respectively. There are a total of 19 common compounds. The main volatile constituents in PCR, PCRV and HP PCR - PCRV are D-limonene, γ-terpinene, linalool and α-farnesene.