Sequence Analysis of Mitochondrial DNA D-loop Region in Xinjiang Goose

新疆鹅线粒体DNA D-loop区序列分析(英文)

[Objective] The sequences of mitochondrial DNA D-loop region of Xinjiang Goose with three different colors of plumage were analyzed in order to study the genetic diversity of Xinjiang Goose, as well as the phylogeny and evolution. [Method] Ten geese were selected randomly from the core populations of grey-, mosaic- and white-plumaged Xinjiang Goose respectively with a total number of thirty as experi- mental materials, of which the blood samples were collected from the largest vein under the wing （brachial vein） for DNA extraction. Sequences of mitochondrial DNA D-loop regions were determined using DNA sequencing technology to analyze the polymorphism. In addition, the genetic distances among different populations were estimated through the comparison with the reference sequences. [Resull] The con- tents of A, G, C and T nucleotides in the D-loop region of Xinjiang Goose were 28.85%, 17.05%, 25.38% and 28.72%, respectively. The average haplotype diversity and nucleotide diversity of Xinjiang Goose were 0.583 and 0.056. Xinjiang Goose and Greylag Goose were clustered into the same group. [Conclusion] The results showed that Xinjiang Geese with three different colors of plumage all descend from Greylag Goose （Anser anser）.

[目的]通过线粒体DNAD-loop区序列分析研究不同羽色新疆鹅的遗传多样性和系统进化。[方法]从新疆鹅核心群中随机选取灰羽、花羽、白羽新疆鹅各10只,共30只,作为试验材料,采集其翅下静脉血。运用DNA测序技术对新疆鹅线粒体DNAD-loop区序列进行了测定分析,研究其多态性。通过与参考序列的对比分析,估计群体间的遗传距离。[结果]不同羽色的新疆鹅线粒体DNAD-loop区的A、G、C、T含量分别为28.85%、17.05%、25.38%和28.72%。平均单倍型多样度和核苷酸多样度分别为0.583和0.056。聚类分析结果显示,新疆鹅与灰雁聚为一类。[结论]该研究结果表明不同羽色的新疆鹅均起源于灰雁(Anser anser)。