葡萄籽提取物对脱矿牙本质极限拉伸强度及树脂-牙本质粘接强度的影响

The effect of grape seed extract on the ultimate tensile strength of demineralized dentin and resin-dentin bonding strength

目的:探讨葡萄籽提取物(grape seed extract,GSE)短暂预处理脱矿牙本质对其极限拉伸强度及树脂-牙本质即刻微拉伸粘接强度的影响。方法:以乙醇、丙酮、蒸馏水为溶剂配制不同浓度的GSE溶液,以溶剂为空白对照,无预处理为阴性对照,5%戊二醛为阳性对照,每组样本15例。GSE预处理脱矿牙本质后,测试牙本质极限拉伸强度及树脂-牙本质微拉伸粘接强度,观察粘接断裂面微观形貌并分析断裂模式。结果:GSE预处理组牙本质极限拉伸强度均呈浓度和时间依赖性增加。应用Single Bond 2粘接时,无论何种溶剂,10%或15%GSE预处理组微拉伸粘接强度显著高于空白对照组(P<0.05);而用Prime&Bond NT粘接时,仅15%GSE水溶剂组和10%GSE丙酮溶剂组微拉伸粘接强度显著高于空白对照组(P<0.05)。交联预处理对水和乙醇溶剂的Single Bond 2粘接时微拉伸粘接强度的改善作用比用水和丙酮溶剂的Prime&Bond NT粘接时更为显著。粘接试件断裂均以混合破坏为主,预处理组试件断裂多发生在混合层顶部,而阴性和空白对照组断裂多位于混合层底部。结论:GSE短暂预处理脱矿牙本质可改善其机械性能,增强混合层,并提高树脂-牙本质即刻微拉伸粘接强度。

Objective： To investigate the effect of grape seed extract （GSE） transient pretreatment on the ultimate tensile strength （UTS） of demineralized dentin and resin-dentin microtensile bonding strength （p, TBS）. Methods： Demineralized dentin specimens were randomly divided into different groups（ n = 15）. Specimens pretreated by pure solvents were used as the blank controls and those without pretreatment as the negative controls. Dentin specimens were exposed to the preconditioners after etching, then UTS was as- sessed. After bonded with two different solvent-based adhesives respectively dentin specimens were subjected to p^TBS test and the ex- amination of debonded interface and failure mode analysis. Results： UTS of preconditioned specimens exhibited concentration and time- dependent increase with the preconditioner and the solvent. Pretreatment by GSE preconditioners （at 10% or 15%, either in water or ethanol） significantly improved the p, TBS of Single Bond 2（P 〈0.05）. However, only 15% GSE in water and 10% GSE in acetone groups presented significantly higher μTBS when using Prime ＆ Bond NT（ P 〈 0.05 ）. Mixed failures were predominant in each group. In GSE pretreated specimens, most of the fractures happened at the top of the hybrid layer; while in the non-treated group,it was found at its bottom. Conclusion： Transient pretreatment by GSE-based preconditioners can strengthen the demineralized dentin and enhance its bond to resin.