快速高分离液相色谱/四级杆串联飞行时间质谱分析山楂籽中的主要黄酮成分

Analysis of Flavonoids in Hawthorn Seed using Rapid Resolution Liquid Chromatography-Quadrupole-Time of Flight Mass Spectrometer

采用快速高分离液相色谱/四级杆串联飞行时间质谱(RRLC/Q-TOF MS)联用技术进行山楂籽中的黄酮类化合物分析方法研究.山楂籽黄酮经超声辅助提取后,选用Welch Materials C18柱(250 mm×4.6 mm,5μm),流动相组成分别为0.1%甲酸水溶液和乙腈,梯度洗脱.在正离子模式下,经飞行时间质谱分离并检测了15种黄酮成分.结果表明,快速分离液相-四级杆串联飞行时间质谱联用技术可以快速准确鉴定样品中的黄酮成分.

In the paper, a novel method for flavonoids analysis in Hawthorn seed was investigated using rapid resolution liquid chromatography/quadrupole-time of flight mass spectrometer （RRLC/Q-TOF MS）. The flavonoids compounds from Hawthorn seed were extracted by ultrasound-assisted extraction technology and then separated using Welch Materials Cls column （250 mmx 4.6 mm, 5 μm）. Water containing 0. 1% formic acid and acetonitrile was used as mobile phase. Time-of-flight mass spectrometer （TOF/MS） was applied for qualitative analysis under positive ion mode. Fifteen of the major flavones of hawthorn seed were identified. In conclusion, this method can be used to determine the flavones of Haw- thorn seed rapidly.