Difference Analysis on Contents of Gallic Acid and Catechins of Tea Resources from Yunnan Province

云南茶树资源没食子酸和儿茶素的差异性分析(英文)

[Objective] This study aimed to analyze the difference in the contents of gallic acid and catechins of tea resources from Yunnan Province. [Method] By using high performance liquid chromatography （HPLC）, the contents of gallic acid （GA）, catechins （C）, epicatechin （EC）, epigallocatechin （EGC）, epicatechin gallate （ECG） and epigallocatechin gallate （EGCG） of 121 tea germplasms from the China National Germplasm Tea Repositories （CNGTR） at the Tea Research Institute of Yunnan Academy of Agricultural Sciences （TRIYAAS） were measured. [Result] The content of GA ranged from 0.210% to 1.902%, with an average of 0.834%, explaining rela- tively low GA content among tea germplasms. The content of C ranged from 0.069% to 8.865%, with an average of 1.916%. The content of EC ranged from 0.126% to 2.865%, with an average of 1.112%. The content of EGC ranged from 0.00% to 3.709%, with an average of 0.954%. The content of ECG ranged from 0.739% to 8.957%, with an average of 4.063%. The content of EGCG ranged from 0.819% to 11.77%, with an average of 5.939%. The content of total C ranged from 6.354% to 22.654%, with an average of 14.042%. [Conclusion] There was relatively big difference of catechin contents among different tea resources, indicating that there was plentiful biodiversity of Yunnan tea germplasms. At the same time, three tea germplasms with high epigallocatechin gallate content （≥10%） was selected preliminarily, which would provide important materials for breeding tea cultivars with high EGCG content in the future.

[目的]探讨云南茶树资源中没食子酸和儿茶素含量的差异。[方法]以国家种质勐海茶树资源圃中的121个云南茶树资源为材料,采用高效液相色谱法(HPLC)测定茶叶中的没食子酸(GA)、儿茶素(C)、表儿茶素(EC)、表儿茶素没食子酸酯(ECG)、表没食子酸儿茶素(EGC)及表没食子儿茶素没食子酸酯(EGCG)的含量。[结果]GA含量变幅为0.210%～1.902%,平均0.834%,在茶树中含量较低。C含量变幅为0.069%～8.865%,平均1.916%;EC含量变幅为0.126%～2.865%,平均1.112%;EGC含量变幅为0.00～3.709%,平均0.954%;ECG的含量变幅为0.739%～8.957%,平均4.063%;EGCG含量变幅为0.819%～11.77%,平均5.939%;儿茶素总量含量为6.354%～22.654%,平均14.042%。[结论]资源间儿茶素差异较大,说明云南茶树资源具有丰富的生物多样性。同时,筛选出3份高EGCG含量(≥10%)的特异茶树资源,为高EGCG茶树品种选育提供了重要材料。