大肠癌中CCR7、L-selectin、CD44v6和MMP9表达及其与淋巴转移的关系

Expression of CCR7,L-selectin,CD44v6 and MMP9 in colorectal cancer and their relative with lymphatic metastatic

目的探讨CC趋化因子受体7(CCR7)、L-selectin、CD44v6和MMP9在大肠癌组织中的表达及其与大肠癌淋巴转移的关系。方法采用免疫组化PV 9000两步法检测104例大肠癌组织(大肠癌组)、55例癌旁正常组织(癌旁组)和34例转移灶组织(转移组)中CCR7和L-selectin、CD44v6和MMP9的表达。结果大肠癌组、转移组中CCR7、L-selectin、CD44v6和MMP9阳性率明显高于癌旁组(P<0.05),有淋巴结转移者明显高于无转移者(P<0.05);CCR7与L-selectin、MMP9表达呈正相关(P<0.05);CD44v6与L-selectin、MMP9表达相关;L-selectin与MMP9的表达呈正相关。结论 CCR7、L-selectin、CD44v6和MMP9在大肠癌中的表达与大肠癌的发生、淋巴转移有关,它们可能共同参与了大肠癌发生及淋巴结转移过程。

Purpose To investigate the expression of chemokine receptor 7 （CCR7）, adhesion molecule L-selectin, CD44v6 and MMP9 in human eoloreetal carcinoma, and analyze their correlation with lymph node metastasis. Methods The expression of CCR7, L-selectin, CD44v6 and MMP9 were tested by immunohistochemical method in 104 cases of colorectal carcinoma specimens （ eolorectal carcinoma group）, 55 cases of normal intestinal mucosa adjacent to carcinoma （ adjacent group） and 34 cases of metastatic tumor tissue （ metastasis group）. Results The positive rate of CCR7, L-seleetin, CD44v6 and MMP9 expression in the coloreetal carcinoma group and metastatic tumor tissue were significantly higher than that in adjacent group and metastasis group （ P 〈 0. 05 ）. The expression of CCR7, L-selectin, CD44v6 and MMP9 in the tissue with lymph node metastasis was significantly higher than that in the tissue without lymph node metastasis （ P 〈 0. 05 ）. There was significantly positive correlation between expression of CCR7 and L-selectin, CCR7 and MMP9, CD44v6 and L-selectin, CD44v6 and MMP9, L-selectin and MMP9 （P 〈 0. 05）. Conclusions The expression of CCR7, L- selectin, CDd4v6 and MMP9 are closely associated with development and metastasis of the colorectal carcinoma. They maybe partici- pate together in the carcinogenesis and lymph node metastasis of colorectal carcinoma.