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Epidot-酶联免疫斑点检测方法应用于结核诊断的初步评价 被引量：4

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摘要 目的:初步评价自行研发的结核分枝杆菌抗原特异性γ-干扰素酶联免疫斑点(Epidot-ELISPOT)检测方法应用于结核病诊断的价值。方法:应用自行研发的Epidot-ELISPOT检测方法及进口T-SPOT.TB试剂盒,检测60份结核病例和75份健康人群的外周血结核特异性γ-干扰素释放水平[斑点形成细胞(spot forming cells,SFCs)]。结果:ELISPOT方法与T-SPOT.TB检测方法对结核感染人群中的敏感性分别为81.67%、90.00%,特异度分别为94.67%、94.67%,Epidot-ELISPOT的敏感度与T-SPOT.TB比较(χ2=1.313,P=0.252),特异性比较(χ2=0.000,P=1.000),差异均无统计学意义。分别用上述两种方法检测肺结核痰阳组和痰阴组形成的SFCs,组间差异均无统计学意义。结论:自行研发的结核分枝杆菌抗原特异性γ-干扰素酶联免疫斑点技术的灵敏度和特异性与T-SPOT.TB检测方法类似,可用于结核感染的辅助诊断。
作者 石利民 王燕 崔红莲 许文炯 史秋磊 乔梦凯 雍玮 孙美艺 丁洁
机构地区 南京市疾病预防控制中心微生物检验科 中国科学院微生物研究所分子病毒和分子免疫室 北京表源生物技术公司研发部
出处 《江苏大学学报（医学版）》 CAS 2012年第5期444-447,451,共5页 Journal of Jiangsu University：Medicine Edition
基金 南京市医学科技发展重大项目(2007年第18号) 南京市卫生局重大科研资助项目(ZKX08020)*:
关键词 结核 酶联免疫斑点实验 T-SPOT Epidot-ELISPOT
分类号 R446.61 [医药卫生—诊断学]
  • 相关文献

参考文献14

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二级参考文献45

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共引文献30

同被引文献48

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  • 2崔会娟,何俊瑛.脑脊液检测诊断结核性脑膜炎[J].河北医科大学学报,2006,27(5):498-500. 被引量：12
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引证文献4

二级引证文献20

江苏大学学报（医学版）
2012年 第5期

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