SENP1和HER-2在胃癌组织中的表达及意义

Protein expression and significance of SENP1 and HER-2 in the gastric cancer

目的:探讨胃癌组织中去类泛素化蛋白酶1(SENP1)的表达与临床病理因素的关系,及其与人表皮生长因子受体-2(HER-2)的表达间的相互关系。方法:采用免疫组织化学染色EnVision方法以兔抗人SENP1抗体和鼠抗人HER-2抗体为一抗,检测100例胃癌组织(胃癌组)和50例胃良性病变组织(对照组)中SENP1和HER-2蛋白的表达。结果:SENP1蛋白在胃癌组织中的阳性率为75%(75/100),对照组阳性率为36%(18/50),二者差异有统计学意义(P<0.01);SENP1的表达与胃癌的淋巴结转移、浸润深度、肿瘤分化程度和临床分期有关(P<0.05)。HER-2蛋白在胃癌组织中的阳性率为45%(45/100),对照组阳性率为38%(19/50),二者差异有统计学意义(P<0.01);HER-2的表达与胃癌的淋巴结转移、浸润深度和临床分期有关(P<0.05)。SENP1蛋白和HER-2蛋白的表达呈正相关(r=0.208,P<0.05)。结论:SENP1、HER-2在胃癌组织中存在着过度表达,它们参与了胃癌的演进;SENP1调节HER-2的表达,在胃癌发生发展中发挥重要作用;SENP1、HER-2的过度表达与胃癌的发生发展有关,可能成为预测胃癌侵袭、转移及预后的重要指标。

Objective： To investigate the expression and significance of SENP1 and HER-2 in the gastric cancer. Methods： EnVision immunohistochemical staining method was used to detect the expression of SENP1 protein and HER-2 protein respectively, in gastric cancer and benign stomach lesion. Results： The SENP1 positive expression rate in gastric cancer was 75% （75/100）, and in benign stomach lesion was 36% （18/50）. there were significant relationship between gastric cancer and benign stomach lesions （P〈0.01）. and the expression of the SENP1 was correlated with the degree of infiltration and differentiation, lymph node metastasis and TNM stage of gastric cancer （P〈0.05）. The HER-2 positive expression rate in gastric cancer was 45% （45/100）, and in benign stomach lesions was 38% （19/50）. there were significant relationship between gastric cancer and benign stomach lesions（P〈0.01 ）. and the expression of the Her-2 was correlated with the degree of infiltration, lymph node metastasis and TNM stage of gastric cancer （P〈0.05）. The expression of SENP1 and HER-2 was positive related （r=0.208, P〈0.05）. Conclusion： It was pointed out that SENP1 and HER-2 took part in process of gastric cancer in occurrence and development, and was related to carcinomas in advance and lymph node metastasis.