苦杏仁甙化学发光生物传感器

A Chemiluminescence Biosensor for Amygdalin

将具有分子识别功能的β－葡萄糖甙酶和能进行换能反应的Ｌｕｍｉｎｏｌ分别固定在壳质胺和大孔阴离子交换剂的柱中，组成流动注射系统。苦杏仁甙在β－葡萄糖甙酶催化下分解生成的ＣＮ＾－（分子识别反应）与溶解氧反应生成超氧阴离子自由基，继而同Ｌｕｎｉｎｌ反应产生化学发光（换能反应）。这一新型生物传感器的化学发光强度与苦杏杜甙量在１～２００ｕｇ之间呈良好线性关系，检出限为０．３ｕｇ，相对标准偏差为３．１％。

A chemiluminescence biosensor responding to amygdalin was prepared by covalently coupling β-glucosidase to the controlled pore glass as a molecular recognition element and electrosatically immoblizing luminol on the ion exchange resin as a transduction element in a flow injection system. The analyte was injected into a continuous stream of simple medium flowing through an enzyme reactor containing β-glucosidase to produce the cyanide, which reacted with the soluble O2 to produce superoxide anion O2. The luminol was eluted by NaOH solution from the ion exchange column, then reacted with superoxide anion O2 to produce chemiluminescence. The linear range was from 1 .0 μg to 200 μg, the detection limit was 0. 3 μg of amygdailn. The sensor was stable in 6 months and it has been applied successfully to the determination of amygdalin in amygdalloid nucleus.