黄芪对机体免疫力影响的谱效关系研究

Spectrum-effect Relationship in Effect of Improving Immunity of Astragali Radix

目的:阐明黄芪的高效液相色谱(HPLC)图谱与提高机体免疫力药效的相关性,表征黄芪药材的药效物质基础。方法:进行小鼠碳粒廓清试验,用灰色关联统计方法将HPLC图谱的各组分峰与提高机体免疫力药效数据相关联,研究谱效相关性,找出黄芪提高机体免疫力作用的物质基础。结果:黄芪提取物在碳粒廓清实验中,水部分组吞噬指数最大,提高免疫力的能力最强,用灰色关联的分析方法,根据关联度大小来确定黄芪药材水部分中以黄芪多糖为代表物质群对提高机体免疫力作用贡献程度。结论:研究黄芪药材HPLC图谱特征及谱效关系,是表征其谱效相关性的有效途径。

Objective：To elaborate the correlation of chromatography fingerprint of Astragali Radix and the efficacy of improving immunity,and express the effective substances foundation.Methods：The water extract was given to irrigation stomach of mice for carbon clearance experiment.Associated the peak area of each common peak from HPLC fingerprint and the date of improving immunity,and studied the correlation between fingerprint and efficacy and found out the effective substances foundation of improving immunity in the method of gray correlation analysis.Results：Polysacharides was the main component of the water extract of Astragali Radix,which could improve immunity function.In carbon clearance experiment,the phagocytic index of the mice with water extract was the largest,and the ability to enhance immunity was the strongest.Grey correlation analysis was used to evaluate the correlation of the effect of improving immunity and the component of the water extract of Astragali Radix.Conclusion：It is effective to study the fingerprint and the correlation of fingerprint and the efficacy of traditional chinese medicine for expressing its correlation.